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Zygotes

Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection

Kohtaro Morita, Shunya Ihashi, Eiichi Okamura, Kazuya Goto, Toru Yoshihara, Arata Honda, Masatsugu Ema, Masahide Asano

published 17 Feb 2026

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Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age

Naomi Nakagata, Satohiro Nakao, Nobuyuki Mikoda, Katsuma Yamaga, Yoshiko Nakagawa, Tetsushi Sakuma, Takashi Yamamoto, Toru Takeo

published 08 Sep 2025

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Induction of autophagy in one-cell stage somatic cell nuclear transfer embryos improves preimplantation embryonic development in goat species

Nasrin Mahvash, Reza Moradi-Hajidavaloo, Farnoosh Jafarpour, Mehdi Hajian, Mohsen Rahimi, Nafiseh Sanei Ata-abadi, Marjan Sadeghi, Mohammad Hossein Nasr-Esfahani

published 28 Apr 2025

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A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

published 27 Mar 2025

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Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid

Verónica M. Negrón-Pérez, Abdullah Al Naib, Abigail L. Zezeski, Victoria L. McCracken-Harlow, George A. Perry, Alan D. Ealy, Michelle L. Rhoads

published 07 Feb 2025

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Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa

Hisayoshi Nozaki, Ryo Matsuzaki, Koichi Shimotori, Noriko Ueki, Wirawan Heman, Wuttipong Mahakham, Haruyo Yamaguchi, Yuuhiko Tanabe, Masanobu Kawachi

published 23 Sep 2024

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Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice

Geraldine Schlapp, María Noel Meikle, Jorge Luis Pórfido, Alejo Menchaca, Martina Crispo

published 09 Jul 2024

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Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts

Carmen Huayhua, Misael Rodríguez, Jhorjhi Vega, Mario Briones, Lleretny Rodriguez-Alvarez, Edwin Mellisho

published 10 Aug 2023

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Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system

Giuliana de Avila Ferronato, Carolina Mônica dos Santos, Paola Maria da S. Rosa, Alessandra Bridi, Felipe Perecin, Flávio Vieira Meirelles, Juliano Rodrigues Sangalli, Juliano Coelho da Silveira

published 21 Apr 2023

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Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage

Tamás Somfai, Seiki Haraguchi, Thanh Quang Dang-Nguyen, Hiroyuki Kaneko, Kazuhiro Kikuchi

published 17 Mar 2023

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GM-CSF perturbs cell identity in mouse pre-implantation embryos

Tim Pock, Katharina Schulte, Stefan Schlatt, Michele Boiani, Verena Nordhoff

published 10 Feb 2022

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Optimised CO₂-containing medium for in vitro culture and transportation of mouse preimplantation embryos without CO₂ incubator

Yasuyuki Kikuchi, Sayaka Wakayama, Daiyu Ito, Masatoshi Ooga, Teruhiko Wakayama

published 23 Dec 2021

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Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1

Katy S. Stoecklein, M. Sofia Ortega, Lee D. Spate, Clifton N. Murphy, Randall S. Prather

published 03 Feb 2021

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