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Vector cloning

Construction of E. coli—Mycobacterium shuttle vectors with a variety of expression systems and polypeptide tags for gene expression in mycobacteria

Surya Pratap Seniya, Priya Yadav, Vikas Jain

Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and Essentiality of M. smegmatis MSMEG_1556

Shuang Li, Jian Kang, [ ... ], Yufang Ma

2A and the Auxin-Based Degron System Facilitate Control of Protein Levels in Plasmodium falciparum

Andrea Kreidenweiss, Annika V. Hopkins, Benjamin Mordmüller

High Yield Production and Refolding of the Double-Knot Toxin, an Activator of TRPV1 Channels

Chanhyung Bae, Jeet Kalia, [ ... ], Jae Il Kim

Expansion of the Gateway MultiSite Recombination Cloning Toolkit

Harold K. Shearin, Alisa R. Dvarishkis, Craig D. Kozeluh, R. Steven Stowers

Splice Isoforms of the Polyglutamine Disease Protein Ataxin-3 Exhibit Similar Enzymatic yet Different Aggregation Properties

Ginny Marie Harris, Katerina Dodelzon, [ ... ], Henry L. Paulson

Functional Characterization of the Incomplete Phosphotransferase System (PTS) of the Intracellular Pathogen Brucella melitensis

Marie Dozot, Sandrine Poncet, [ ... ], Jean-Jacques Letesson

Isolation, cloning and expression of CCA1 gene in transgenic progeny plants of Japonica rice exhibiting altered morphological traits

Ashok Chaudhury, Anita Devi Dalal, Nayan Tara Sheoran

An Infinitely Expandable Cloning Strategy plus Repeat-Proof PCR for Working with Multiple shRNA

Glen John Mcintyre, Jennifer Lynne Groneman, Anna Tran, Tanya Lynn Applegate

Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Wataru Fujii, Kiyoshi Kano, Koji Sugiura, Kunihiko Naito

Construction and Application of an Inducible System for Homogenous Expression Levels in Bulk Cell Lines

Jun Yu, Helena Mϋller, [ ... ], Carsten Müller-Tidow

The GPI Anchor Signal Sequence Dictates the Folding and Functionality of the Als5 Adhesin from Candida albicans

Mohammad Faiz Ahmad, Bhawna Yadav, [ ... ], Sneha Sudha Komath

An Alternative Method to Facilitate cDNA Cloning for Expression Studies in Mammalian Cells by Introducing Positive Blue White Selection in Vaccinia Topoisomerase I-Mediated Recombination

Hiroshi Udo

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