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Vector cloning

Optimisation of Bioluminescent Reporters for Use with Mycobacteria

Nuria Andreu, Andrea Zelmer, [ ... ], Siouxsie Wiles

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter

Jane Yuxia Qin, Li Zhang, [ ... ], Bruce T. Lahn

Generation of Human CEACAM1 Transgenic Mice and Binding of Neisseria Opa Protein to Their Neutrophils

Angel Gu, Zhifang Zhang, [ ... ], John E. Shively

The Transposon Galileo Generates Natural Chromosomal Inversions in Drosophila by Ectopic Recombination

Alejandra Delprat, Bàrbara Negre, Marta Puig, Alfredo Ruiz

Phenylalanine-Rich Peptides Potently Bind ESAT6, a Virulence Determinant of Mycobacterium tuberculosis, and Concurrently Affect the Pathogen's Growth

Krishan Kumar, Megha Tharad, [ ... ], Anand Ranganathan

Single Tube, High Throughput Cloning of Inverted Repeat Constructs for Double-Stranded RNA Expression

Brian Hauge, Christopher Oggero, [ ... ], Fenggao Dong

The 3′ Region of the Chicken Hypersensitive Site-4 Insulator Has Properties Similar to Its Core and Is Required for Full Insulator Activity

Paritha I. Arumugam, Fabrizia Urbinati, [ ... ], Punam Malik

Caenorhabditis elegans BAH-1 Is a DUF23 Protein Expressed in Seam Cells and Required for Microbial Biofilm Binding to the Cuticle

Kevin Drace, Stephanie McLaughlin, Creg Darby

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Eric Campeau, Victoria E. Ruhl, [ ... ], Paul D. Kaufman

Construction and Application of an Inducible System for Homogenous Expression Levels in Bulk Cell Lines

Jun Yu, Helena Mϋller, [ ... ], Carsten Müller-Tidow

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Jiayuan Quan, Jingdong Tian

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

Cycle Inhibiting Factors (CIFs) Are a Growing Family of Functional Cyclomodulins Present in Invertebrate and Mammal Bacterial Pathogens

Grégory Jubelin, Carolina Varela Chavez, [ ... ], Eric Oswald

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