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Vector cloning

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

Jin Hee Kim, Sang-Rok Lee, [ ... ], Seok-Yong Choi

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter

Jane Yuxia Qin, Li Zhang, [ ... ], Bruce T. Lahn

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet

A Modular Cloning System for Standardized Assembly of Multigene Constructs

Ernst Weber, Carola Engler, [ ... ], Sylvestre Marillonnet

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Eric Campeau, Victoria E. Ruhl, [ ... ], Paul D. Kaufman

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

Athanasios Lampropoulos, Zoran Sutikovic, [ ... ], Joachim Forner

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Jiayuan Quan, Jingdong Tian

Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli

Ana Paula Jacobus, Jeferson Gross

Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

Changlin Fu, William P. Donovan, [ ... ], Robert H. Cole

A Practical Comparison of Ligation-Independent Cloning Techniques

Julian Stevenson, James R. Krycer, Lisa Phan, Andrew J. Brown

A Gateway MultiSite Recombination Cloning Toolkit

Lena K. Petersen, R. Steven Stowers

A Practical Approach to T-Cell Receptor Cloning and Expression

Sébastien Wälchli, Geir Åge Løset, [ ... ], Johanna Olweus

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