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Transposon mutagenesis

Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM)

Antoine Champie, Amélie De Grandmaison, Simon Jeanneau, Frédéric Grenier, Pierre-Étienne Jacques, Sébastien Rodrigue

published 11 Apr 2023

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A streamlined method for transposon mutagenesis of Rickettsia parkeri yields numerous mutations that impact infection

Rebecca L. Lamason, Natasha M. Kafai, Matthew D. Welch

published 03 May 2018

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Leptospiral flagellar sheath protein FcpA interacts with FlaA2 and FlaB1 in Leptospira biflexa

Yuya Sasaki, Akihiro Kawamoto, Hajime Tahara, Kie Kasuga, Ryoichi Sato, Makoto Ohnishi, Shuichi Nakamura, Nobuo Koizumi

published 10 Apr 2018

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MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation

Ryosuke Kadoya, Ken’ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi

published 03 Jun 2015

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Atypical Role for PhoU in Mutagenic Break Repair under Stress in Escherichia coli

Janet L. Gibson, Mary-Jane Lombardo, Ildiko Aponyi, Diana Vera Cruz, Mellanie P. Ray, Susan M. Rosenberg

published 11 May 2015

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Molecular Characterization of a Multidrug Resistance IncF Plasmid from the Globally Disseminated Escherichia coli ST131 Clone

Minh Duy Phan, Brian M. Forde, Kate M. Peters, Sohinee Sarkar, Steven Hancock, Mitchell Stanton-Cook, Nouri L. Ben Zakour, Mathew Upton, Scott A. Beatson, Mark A. Schembri

published 15 Apr 2015

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Identification of Listeria monocytogenes Determinants Required for Biofilm Formation

Almaris N. Alonso, Kyle J. Perry, James M. Regeimbal, Patrick M. Regan, Darren E. Higgins

published 17 Dec 2014

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Clonal Expansion Analysis of Transposon Insertions by High-Throughput Sequencing Identifies Candidate Cancer Genes in a PiggyBac Mutagenesis Screen

Roland H. Friedel, Caroline C. Friedel, Thomas Bonfert, Ruijin Shi, Roland Rad, Philippe Soriano

published 05 Aug 2013

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Mab_3168c, a Putative Acetyltransferase, Enhances Adherence, Intracellular Survival and Antimicrobial Resistance of Mycobacterium abscessus

Sheng-Hui Tsai, Gwan-Han Shen, Chao-Hsiung Lin, Jiue-Ru Liau, Hsin-Chih Lai, Shiau-Ting Hu

published 28 Jun 2013

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Transposon Mutagenesis in Bifidobacterium breve: Construction and Characterization of a Tn5 Transposon Mutant Library for Bifidobacterium breve UCC2003

Lorena Ruiz, Mary O’Connell Motherway, Noreen Lanigan, Douwe van Sinderen

published 30 May 2013

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A Genetic Screen Using the PiggyBac Transposon in Haploid Cells Identifies Parp1 as a Mediator of Olaparib Toxicity

Stephen J. Pettitt, Farah L. Rehman, Ilirjana Bajrami, Rachel Brough, Fredrik Wallberg, Iwanka Kozarewa, Kerry Fenwick, Ioannis Assiotis, Lina Chen, James Campbell, Christopher J. Lord, Alan Ashworth

published 25 Apr 2013

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piggyBac Transposon Somatic Mutagenesis with an Activated Reporter and Tracker (PB-SMART) for Genetic Screens in Mice

Sean F. Landrette, Jonathan C. Cornett, Thomas K. Ni, Marcus W. Bosenberg, Tian Xu

published 21 Oct 2011

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Screen for Localized Proteins in Caulobacter crescentus

Jay H. Russell, Kenneth C. Keiler

published 12 Mar 2008

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