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Transformation associated recombination

Frequent sgRNA-barcode recombination in single-cell perturbation assays

Shiqi Xie, Anne Cooley, [ ... ], Gary C. Hon

TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

Athanasios Niarchos, Anastasia Siora, [ ... ], Konstantinos Poulas

An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome

Yanlong Wei, Pingping Deng, [ ... ], Hongqing Fang

Exploring Lactobacillus reuteri DSM20016 as a biocatalyst for transformation of longer chain 1,2-diols: Limits with microcompartment

Lu Chen, Rajni Hatti-Kaul

Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

Joakim Näsvall

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene

Salwa Sabet, Marina A. George, [ ... ], Taher A. Salaheldin

Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli

Young Shin Ryu, Sathesh-Prabu Chandran, Kyungchul Kim, Sung Kuk Lee

Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1

Yao Lan, Xiaohuan Zhang, [ ... ], Zhemin Zhou

Both IIC and IID Components of Mannose Phosphotransferase System Are Involved in the Specific Recognition between Immunity Protein PedB and Bacteriocin-Receptor Complex

Wanli Zhou, Guohong Wang, [ ... ], Yanling Hao

Construction of Marker-Free Transgenic Strains of Chlamydomonas reinhardtii Using a Cre/loxP-Mediated Recombinase System

Yuki Kasai, Shigeaki Harayama

Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

Moushree Pal Roy, Deepika Mazumdar, [ ... ], Shilpi Ghosh

Engineering of a Bacillus amyloliquefaciens Strain with High Neutral Protease Producing Capacity and Optimization of Its Fermentation Conditions

Hui Wang, Lian Yang, [ ... ], Bin Yao

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