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Synthetic biology

A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system

Rodrigo Moreno-Campos, Eileen W. Singleton, Rosa A. Uribe

Identifying widespread and recurrent variants of genetic parts to improve annotation of engineered DNA sequences

Matthew J. McGuffie, Jeffrey E. Barrick

Proinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2

Tom Cremer, Hanneke Hoelen, [ ... ], Emmanuel J. H. J. Wiertz

Protocol for the establishment of a serine integrase-based platform for functional validation of genetic switch controllers in eukaryotic cells

Marco A. de Oliveira, Lilian H. Florentino, [ ... ], Elibio Rech

Non-replicative phage particles delivering CRISPR-Cas9 to target major bla_CTX-M variants

Naiyaphat Nittayasut, Teerapong Yata, [ ... ], Pattrarat Chanchaithong

CRISPR-Cas9 genome and long non-coding RNAs as a novel diagnostic index for prostate cancer therapy via liposomal-coated compounds

Mai O. Kadry, Rehab M. Abdel-Megeed

Evaluation of Peregrinus maidis transformer-2 as a target for CRISPR-based control

Yu-Hui Wang, Dina Espinoza Rivera, William Klobasa, Marcé D. Lorenzen

An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9

Mohammad J. Hossain, Tamara J. O’Connor

Comparative evaluation of bioinformatic tools for virus-host prediction and their application to a highly diverse community in the Cuatro Ciénegas Basin, Mexico

Alejandro Miguel Cisneros-Martínez, Ulises E. Rodriguez-Cruz, [ ... ], Valeria Souza

Predicting CRISPR-Cas12a guide efficiency for targeting using machine learning

Aidan O’Brien, Denis C. Bauer, Gaetan Burgio

Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae

Nina Alperovich, Benjamin M. Scott, David Ross

Functional analysis of Collagen 17a1: A genetic modifier of junctional epidermolysis bullosa in mice

Thomas J. Sproule, Robert Y. Wilpan, [ ... ], John P. Sundberg

Performance characteristics and operational feasibility assessment of a CRISPR based tata MD CHECK diagnostic test for SARS-CoV-2 (COVID-19)

Shubhada Shenai, Gita Nataraj, [ ... ], Sarabjit S. Chadha

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