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RNA extraction

Evaluation of RNA extraction and rRNA depletion protocols for RNA-Seq in eleven edible seaweed species from brown, red, and green algae

Rob J. Dekker, Wim A. Ensink, Marina F. van Olst, Selina M. van Leeuwen, Wim C. de Leeuw, Martijs J. Jonker, Timo M. Breit

published 02 Jan 2026

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Development of a competition assay to assess the in vitro fitness of dengue virus serotypes using an optimized serotype-specific qRT-PCR

Anne-Fleur Griffon, Loeïza Rault, Clément Tanvet, Etienne Simon-Lorière, Myrielle Dupont-Rouzeyrol, Catherine Inizan

published 15 Dec 2025

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How much genetic information in RNA form can be protected by a CCMV virus-like particle?

Ana Luisa Duran Meza, Sophie-Christine Porak, Abigail Chapman, Charles M. Knobler, William M. Gelbart

published 10 Dec 2025

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Comparing RNA extraction protocols from formalin-fixed paraffin-embedded microcore samples

Maxime Golias, Zuzana Krupova, Pierre Defrenaix, Marie-Françoise Heymann, Dominique Heymann

published 08 Dec 2025

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Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A. Ashley, Audrey Dubot-Pérès

published 18 Aug 2025

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Characterization of an unusual carlavirus-like RNA from papaya (Carica papaya) lacking essential genes

Milenka Vera, Raúl Cifuentes, Ronan Keener, Neil Olszewski, Robert A. Alvarez-Quinto, Diego F. Quito-Avila

published 08 Aug 2025

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Novel strains of Tomato Spotted Wilt Orthotospovirus (TSWV) are transmitted by western flower thrips in a context-specific manner

Senthilraja Chinnaiah, Arinder K. Arora, Kiran R. Gadhave

published 10 Jul 2025

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RNA degradation patterns in cardiac tissues kept at different time intervals and temperatures before RNA sequencing

Stine Bøttcher Jacobsen, Jacob Tfelt-Hansen, Morten Holdgaard Smerup, Niels Morling, Jeppe Dyrberg Andersen

published 15 May 2025

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Dose response comparison of Nipah virus strains Malaysia and Bangladesh in hamsters exposed by the intranasal or intraperitoneal route

Sara C. Johnston, Ju Qiu, Sarah L.W. Norris, Rekha Panchal, Elizabeth M. Punger, Melissa Teague, Joshua L. Moore, David N. Dyer, Ondraya M. Frick, Stephen C. Stevens, Jimmy Fiallos, Harold L. Mills, Eugene L. Blue, Willie B. Sifford, Trevor McCarson, Amanda Casselman, Jonathan D. Latty, Kathleen E. Huie, Ryan Adolphi, Aysegul Nalca

published 12 May 2025

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Infectious potential and circulation of SARS-CoV-2 in wild rats

Kevyn Beissat, Virginie Lattard, Evelyne Picard-Meyer, Ambre Fafournoux, Sionfoungo Daouda Soro, Alexandre Servat, Françoise Vincent-Hubert, Franck Boué, Nolan Chatron, Elodie Monchâtre-Leroy, Marine Wasniewski

published 12 May 2025

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Identification of stable reference genes for qRT-PCR in Stropharia rugosoannulata using mRNA-sequencing data

Xiaohua Zhang, Xuesong Li, Rong Hua, Yuan Fang, Tingsong Yue, Jianying Li, Yuxun Lu, Wansong Yue, Zhanghui Gao, Shaoxiong Liu, Dafeng Sun

published 07 May 2025

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Reliable RNA-seq analysis from FFPE specimens as a means to accelerate cancer-related health disparities research

Mitchell J. Frederick, Dannelys Perez-Bello, Pedram Yadollahi, Patricia Castro, Alan Frederick, Andrew Frederick, Rashid A. Osman, Fonma Essien, Imelda Yebra, Ashley Hamlin, Thomas J. Ow, Heath D. Skinner, Vlad C. Sandulache

published 21 Apr 2025

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mRNA extracted from frozen buffy coat samples stored long term in tubes with no RNA preservative shows promise for downstream sequencing analyses

Erik Bovinder Ylitalo, Linda Vidman, Sophia Harlid, Bethany Van Guelpen

published 19 Mar 2025

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