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Reverse transcription

Clinical evaluation of the SD Biosensor SARS-CoV-2 saliva antigen rapid test with symptomatic and asymptomatic, non-hospitalized patients

Zsofia Igloi, Jans Velzing, Robin Huisman, Corine Geurtsvankessel, Anoushka Comvalius, Jeroen IJpelaar, Janko van Beek, Roel Ensing, Timo Boelsums, Marion Koopmans, Richard Molenkamp

published 22 Dec 2021

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Primer extension coupled with fragment analysis for rapid and quantitative evaluation of 5.8S rRNA isoforms

Giulia Venturi, Federico Zacchini, Cinzia Lucia Vaccari, Davide Trerè, Lorenzo Montanaro

published 21 Dec 2021

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The use of HRM shifts in qPCR to investigate a much neglected aspect of interference by intracellular nanoparticles

Natasha M. Sanabria, Mary Gulumian

published 07 Dec 2021

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Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique

Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada

published 29 Nov 2021

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MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB

Jingjing Liu, Yongsheng Hou, Lili Lin, Nannan Yu, Yanyan Zhang

published 18 Nov 2021

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Diagnostic performance and clinical implications of rapid SARS-CoV-2 antigen testing in Mexico using real-world nationwide COVID-19 registry data

Omar Yaxmehen Bello-Chavolla, Neftali Eduardo Antonio-Villa, Luisa Fernández-Chirino, Enrique C. Guerra, Carlos A. Fermín-Martínez, Alejandro Márquez-Salinas, Arsenio Vargas-Vázquez, Jessica Paola Bahena-López

published 31 Aug 2021

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The combination of the NS5A and cyclophilin inhibitors results in an additive anti-HCV inhibition in humanized mice without development of resistance

Michael Bobardt, Christina M. Ramirez, Marc M. Baum, Daren Ure, Robert Foster, Philippe A. Gallay

published 20 May 2021

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Ultra-precise quantification of mRNA targets across a broad dynamic range with nanoreactor beads

Ivan Francisco Loncarevic, Susanne Toepfer, Stephan Hubold, Susanne Klingner, Lea Kanitz, Thomas Ellinger, Katrin Steinmetzer, Thomas Ernst, Andreas Hochhaus, Eugen Ermantraut

published 18 Mar 2021

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Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yee Ling Lau, Ilyiana binti Ismail, Nur Izati binti Mustapa, Meng Yee Lai, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Maria Kahar Bador Abdul Kahar, Jennifer Chong, Pik Pin Goh

published 06 Jan 2021

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Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification

Johannes W. P. Kuiper, Timo Baade, Marcel Kremer, Ramon Kranaster, Linda Irmisch, Marcus Schuchmann, Johannes Zander, Andreas Marx, Christof R. Hauck

published 02 Nov 2020

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Translesion synthesis by AMV, HIV, and MMLVreverse transcriptases using RNA templates containing inosine, guanosine, and their 8-oxo-7,8-dihydropurine derivatives

Madeline M. Glennon, Austin Skinner, Mara Krutsinger, Marino J. E. Resendiz

published 28 Aug 2020

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Statistical differences resulting from selection of stable reference genes after hypoxia and hypothermia in the neonatal rat brain

Martín Bustelo, Martín A. Bruno, César F. Loidl, Manuel Rey-Funes, Harry W. M. Steinbusch, Antonio W. D. Gavilanes, D. L. A. van den Hove

published 21 May 2020

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Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays

Samuel Long, Brian Berkemeier

published 14 May 2020

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