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Purification techniques

Enzymatic properties of a L-asparaginase without secondary glutaminase activity from Streptomyces scabrisporus

Ricardo Rodríguez-Vargas, Francisca Villanueva-Flores, María Fernanda Gutiérrez-Chávez, Carlos Medrano-Villagómez, Andrés Zárate-Romero, Alejandro Huerta-Saquero

published 07 Nov 2025

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Glutamate dehydrogenase from Pantoea ananatis: A new bacterial enzyme with dual coenzyme specificity

Maria S. Kharchenko, Victoria S. Skripnikova, Julia G. Rostova, Natalia P. Zakataeva

published 19 Aug 2025

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Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A. Ashley, Audrey Dubot-Pérès

published 18 Aug 2025

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Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome

Elizabeth P. Anim, Justin Mezzanotte, Siwei Chu, Ursula Stochaj

published 14 Aug 2025

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Enzymatic characterization and polyurethane biodegradation assay of two novel esterases isolated from a polluted river

Arianna Soto-Hernández, Luis Felipe Muriel-Millán, Adolfo Gracia, Alejandro Sánchez-Flores, Liliana Pardo-López

published 23 Jul 2025

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A test method for selecting suitable cleaning indicators for routine cleaning monitoring on a washer-disinfector in a central sterile supply department

Juan Zhou, Wei Guo, Dongling Liu, Jianrong Li, Caixia Yang, Ying Wang, Xiaoyi Huang

published 01 Jul 2025

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Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissues

Yiqiao Li, Mariana Polychronopoulou, Ine Boonen, Antonios Fikatas, Sophie Gryseels, Anne Laudisoit, Joelle Gouy de Bellocq, Bram Vrancken, Gkikas Magiorkinis, Philippe Lemey, Magda Bletsa

published 30 May 2025

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An inhibitor-free, versatile, fast, and cheap precipitation-based DNA purification method

Zhe F. Tang, David R. McMillen

published 08 Apr 2025

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The transformation pathways and optimization of conditions for preparation minor ginsenosides from Panax notoginseng root by the fungus Aspergillus tubingensis

Fei-Xing Li, Dong-Mei Lin, Jin Yang, Xiu-Ming Cui, Xiao-Yan Yang

published 03 Mar 2025

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Identification of interaction partners of outer inflammatory protein A: Computational and experimental insights into how Helicobacter pylori infects host cells

Sümeyye Akcelik-Deveci, Elif Kılıç, Nesteren Mansur-Ozen, Emel Timucin, Yaren Buyukcolak, Sinem Oktem-Okullu

published 29 Oct 2024

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An expeditious and facile method of amyloid beta (1–42) purification

Md. Aminul Haque, Il Seon Park

published 11 Jul 2024

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TIR domains of TLR family-from the cell culture to the protein sample for structural studies

Vladislav A. Lushpa, Marina V. Goncharuk, Irina A. Talyzina, Alexander S. Arseniev, Eduard V. Bocharov, Konstantin S. Mineev, Sergey A. Goncharuk

published 05 Jul 2024

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Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences

Jia Wei Siau, Asim Azhar Siddiqui, Sze Yi Lau, Srinivasaraghavan Kannan, Sabrina Peter, Yingying Zeng, Chandra Verma, Peter Droge, John F. Ghadessy

published 13 Feb 2024

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