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Plasmid construction

A new mechanism regulating microglial NLRP3 inflammasome: FMR1 mediates NLRP3 mRNA stability

Qian Deng, Qianqian Bai, Yang Yang, Wenchao Tang, Fangfei Liu, Shumin Zhang, Hao Wang, Zhehua Xing, Chi Zhang, Yanhui Yang, Qizhi Fu, Hua Fan

published 20 Feb 2026

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Application of NMRK immobilized enzymes in the synthesis of NMN

Shan Yang, Ting Xiang, Jinchun Qian, Wei Sun, Wenjun Liu, Nengwen Yi, Zheng-Hong Qin

published 19 Feb 2026

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Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection

Kohtaro Morita, Shunya Ihashi, Eiichi Okamura, Kazuya Goto, Toru Yoshihara, Arata Honda, Masatsugu Ema, Masahide Asano

published 17 Feb 2026

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A novel approach towards a histone replacement system in Tetrapods

Maximilian Pfisterer, Amelie Pritz, Anna Parry, M. Lienhard Schmitz

published 10 Feb 2026

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Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions

Chamika De Silva, Brian A. Kelch, Dannel McCollum

published 02 Feb 2026

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Carbon nanotube and carbon dot mediated plasmid DNA delivery in cowpea leaves

Merve Saglam, Nikolaos Tsakirpaloglou, Aya Bridgeland, Robert Miller, Michael J. Thomson, Endang M. Septiningsih

published 27 Jan 2026

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HEDGES co-prevents both SARS-CoV-2 and pandemic influenza infection in mice by rapid, durable co-production of twelve different anti-pandemic monoclonal antibodies

Marissa Mack, Alice Ye, Sarah Ursu, Ryan Ice, Liliana Soroceanu, Stan Shoor, Sean McAllister, Tim Heath, Chakkrapong Handumrongkul, Robert Debs

published 23 Jan 2026

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Deciphering the pathogenicity of three NKX2-1 variants in ultra-severe forms of childhood interstitial lung disease

Yohan David Soreze, Tifenn Desroziers-Louedec, Aurore Carré, Farah Diab, Aphrodite Daskalopoulou, Julie Starck, Valérie Nau, Marie Legendre, Sonia-Athina Karabina, Véronique Houdouin, Aurore Coulomb-L’herminé, Camille Louvrier, Nadia Nathan

published 19 Dec 2025

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The rsmA mutant from Pseudomonas aeruginosa ID4365 is a non-virulent strain that is suitable for pyocyanin and phenazine-1-carboxylic acid production

Misael Josafat Fabian Del-Olmo, Luis Fernando Montelongo-Martínez, Gisela Viridiana Villafañe-Barajas, Abigail González-Valdez, Veronica Roxana Flores-Vega, Roberto Rosales-Reyes, Marco Elias Gudiño-Zayas, Thelma Rizo-Pica, Raunel Tinoco-Valencia, Adelfo Escalante, Gloria Soberón-Chávez, Miguel Cocotl-Yañez

published 04 Dec 2025

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Disrupting ribulose-5-phosphate metabolic flux enhances riboflavin production in Escherichia coli BL21(DE3)

Junhui Ying, Jin Lu, Qiming Liu, Yingjie Pan, Xianfeng Bao, Junjun Yin, Bing Fu

published 14 Nov 2025

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Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology

Claire Verkuyl, Ari Belotserkovsky, Thomas Zerbes, Declan Williams, Medha R. Krishnan, Sabrina Zhu, Sophie Grunnesjӧ, Shehab Eid, Cunjie Zhang, Wenda Zhao, Leo Xu, Eleanore Lin, Teaghan O’Shea, Benjamin Draper, Andreas Jungman, Patrick Most, Gerold Schmitt-Ulms

published 07 Nov 2025

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One IV HEDGES DNA vector administration encoding hGLA or hGH produces durable hGLA and hGH serum lvels in immunocompetent mice

Alice Ye, Marissa Mack, Sarah Ursu, Stephen Chmura, Robert Steiner, Tim Heath, Chakkrapong Handumrongkul, Robert Debs

published 31 Oct 2025

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One SQ HEDGES DNA vector only dose produces durable hGLA or anti-SARS-CoV-2 mAb therapeutic serum protein levels

Alice Ye, Marissa Mack, Ryan Ice, Tim Heath, Elof Ericksson, Stanford Shoor, Robert D. Steiner, Chakkrapong Handumrongkul, Robert Debs

published 31 Oct 2025

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