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Plasmid construction

In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Xinglin Jiang, Jianming Yang, [ ... ], Mo Xian

Transcriptional Activators of Human Genes with Programmable DNA-Specificity

René Geiβler, Heidi Scholze, [ ... ], Jens Boch

Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging

Yoshihiro Nakajima, Tomomi Yamazaki, [ ... ], Yoshihiro Ohmiya

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome

Kathrin Blank, Michael Hensel, Roman G. Gerlach

A Versatile ΦC31 Based Reporter System for Measuring AP-1 and Nrf2 Signaling in Drosophila and in Tissue Culture

Nirmalya Chatterjee, Dirk Bohmann

Evidence Based Selection of Commonly Used RT-qPCR Reference Genes for the Analysis of Mouse Skeletal Muscle

Kristen C. Thomas, Xi Fiona Zheng, [ ... ], Peter J. Houweling

Genetic Incorporation of Unnatural Amino Acids into Proteins in Mycobacterium tuberculosis

Feng Wang, Scott Robbins, [ ... ], Peter G. Schultz

Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR

Andrew Prantner, Dianna Maar

Determination of Plasmid Copy Number Reveals the Total Plasmid DNA Amount Is Greater than the Chromosomal DNA Amount in Bacillus thuringiensis YBT-1520

Chunying Zhong, Donghai Peng, [ ... ], Ming Sun

Characterization of the Metabolically Modified Heavy Metal-Resistant Cupriavidus metallidurans Strain MSR33 Generated for Mercury Bioremediation

Luis A. Rojas, Carolina Yáñez, [ ... ], Michael Seeger

Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase

Yvonne D. Trigoso, Russell C. Evans, William E. Karsten, Lilian Chooback

Ultra-Low Background DNA Cloning System

Kenta Goto, Yukio Nagano

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

Roman J. Szczesny, Katarzyna Kowalska, [ ... ], Andrzej Dziembowski

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