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Plasmid construction

Triose Phosphate Isomerase Deficiency Is Caused by Altered Dimerization–Not Catalytic Inactivity–of the Mutant Enzymes

Markus Ralser, Gino Heeren, [ ... ], Sylvia Krobitsch

Natural DNA Uptake by Escherichia coli

Sunita Sinha, Rosemary J. Redfield

Quantification and isolation of Bacillus subtilis spores using cell sorting and automated gating

Marianna Karava, Felix Bracharz, Johannes Kabisch

Biosynthesis of Vitamin C by Yeast Leads to Increased Stress Resistance

Paola Branduardi, Tiziana Fossati, [ ... ], Danilo Porro

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, [ ... ], Jaffer A. Ajani

Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha

Kimitsune Ishizaki, Ryuichi Nishihama, [ ... ], Takayuki Kohchi

Temporally-Controlled Site-Specific Recombination in Zebrafish

Stefan Hans, Jan Kaslin, Dorian Freudenreich, Michael Brand

Dispersal of Biofilms by Secreted, Matrix Degrading, Bacterial DNase

Reindert Nijland, Michael J. Hall, J. Grant Burgess

Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation

Blaise R. Boles, Matthew Thoendel, Aleeza J. Roth, Alexander R. Horswill

Transformation of Environmental Bacillus subtilis Isolates by Transiently Inducing Genetic Competence

Reindert Nijland, J. Grant Burgess, Jeff Errington, Jan-Willem Veening

Rational Design of a Plasmid Origin That Replicates Efficiently in Both Gram-Positive and Gram-Negative Bacteria

Anton V. Bryksin, Ichiro Matsumura

Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

Evelina Angov, Collette J. Hillier, Randall L. Kincaid, Jeffrey A. Lyon

A Comparative Analysis of Constitutive Promoters Located in Adeno-Associated Viral Vectors

Lkhagvasuren Damdindorj, Sivasundaram Karnan, [ ... ], Hiroyuki Konishi

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