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Nucleases

Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations

Jennifer L. Steele, Richard C. Stevens, Oscar A. Cabrera, Gary J. Bassill, Sabrina M. Cramer, Felipe Guzman, Anthony P. Shuber

published 23 Dec 2020

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A simplified SARS-CoV-2 detection protocol for research laboratories

Sean Paz, Christopher Mauer, Anastasia Ritchie, Janet D. Robishaw, Massimo Caputi

published 18 Dec 2020

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Analysis of RNA yield in extracellular vesicles isolated by membrane affinity column and differential ultracentrifugation

Gilberto Gutiérrez García, Gabriela Galicia García, Jessica Zalapa Soto, Andrea Izquierdo Medina, Mariana Rotzinger-Rodríguez, Gustavo Antonio Casas Aguilar, Cynthia Paola López Pacheco, Álvaro Aguayo, Maria Montserrat Aguilar-Hernandez

published 06 Nov 2020

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RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

John Crissman, Yuhao Lin, Kevin Separa, Madeleine Duquette, Michael Cohen, Candyd Velasquez, Thomas Cujec

published 05 Nov 2020

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RNA-hydrolyzing activity of metallo-β-lactamase IMP-1

Yoshiki Kato, Masayuki Takahashi, Mineaki Seki, Masayuki Nashimoto, Akiko Shimizu-Ibuka

published 30 Oct 2020

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Establishment and characterization of VOA1066 cells: An undifferentiated endometrial carcinoma cell line

Yemin Wang, Valerie Lan Tao, Chae Young Shin, Clara Salamanca, Shary Yuting Chen, Christine Chow, Martin Köbel, Susana Ben-Neriah, David Farnell, Christian Steidl, Jessica N. Mcalpine, C. Blake Gilks, David G. Huntsman

published 14 Oct 2020

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Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study

Mitchell T. Arnold, Jonathan L. Temte, Shari K. Barlow, Cristalyne J. Bell, Maureen D. Goss, Emily G. Temte, Mary M. Checovich, Erik Reisdorf, Samantha Scott, Kyley Guenther, Mary Wedig, Peter Shult, Amra Uzicanin

published 07 Oct 2020

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Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

Mohammad Rubayet Hasan, Faheem Mirza, Hamad Al-Hail, Sathyavathi Sundararaju, Thabisile Xaba, Muhammad Iqbal, Hashim Alhussain, Hadi Mohamad Yassine, Andres Perez-Lopez, Patrick Tang

published 24 Jul 2020

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Combinatorial effects of antibiotics and enzymes against dual-species Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the wound-like medium

Rima Fanaei Pirlar, Mohammad Emaneini, Reza Beigverdi, Maryam Banar, Willem B. van Leeuwen, Fereshteh Jabalameli

published 25 Jun 2020

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Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus

Yugal Raj Bindari, Stephen W. Walkden-Brown, Priscilla F. Gerber

published 22 May 2020

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Intact ribosomes drive the formation of protein quinary structure

Leonard Breindel, Jianchao Yu, David S. Burz, Alexander Shekhtman

published 24 Apr 2020

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SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Derek Cerchione, Katherine Loveluck, Eric L. Tillotson, Fred Harbinski, Jen DaSilva, Chase P. Kelley, Elise Keston-Smith, Cecilia A. Fernandez, Vic E. Myer, Hariharan Jayaram, Barrett E. Steinberg

published 16 Apr 2020

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Stochastic simulation and statistical inference platform for visualization and estimation of transcriptional kinetics

Gennady Gorin, Mengyu Wang, Ido Golding, Heng Xu

published 26 Mar 2020

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