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Nucleases

Chemical nucleases are a robust alternative for RNase H cleavage of human ribosomal RNA

Hagen Wesseling, Dennis Krug, Marvin Wehrheim, Michael W. Göbel, Stefanie Kaiser

published 24 Feb 2025

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Differential impact of divalent metals on native elongating transcript sequencing (NET-seq) protocols for RNA polymerases I and II

Abigail K. Huffines, David A. Schneider

published 13 Feb 2025

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Ruscus aculeatus extract promotes RNase 7 expression through ERK activation following inhibition of late-phase autophagy in primary human keratinocytes

Shigeyuki Ono, Akiko Kawasaki, Kotaro Tamura, Yoshihiko Minegishi, Takuya Mori, Noriyasu Ota

published 03 Dec 2024

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Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method

Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen

published 03 Dec 2024

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The SARS-CoV-2 ORF6 protein inhibits nuclear export of mRNA and spliceosomal U snRNA

Ichiro Taniguchi

published 31 Oct 2024

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Relationship between protein conformational stability and its immunogenicity when administering antigens to mice using adjuvants—Analysis employed the CH₂ domain in human antibodies

Kosuke Oyama, Tadashi Ueda

published 22 Jul 2024

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Ceragenin-mediated disruption of Pseudomonas aeruginosa biofilms

Urszula Wnorowska, Dawid Łysik, Ewelina Piktel, Magdalena Zakrzewska, Sławomir Okła, Agata Lesiak, Jakub Spałek, Joanna Mystkowska, Paul B. Savage, Paul Janmey, Krzysztof Fiedoruk, Robert Bucki

published 12 Feb 2024

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An Open One-Step RT-qPCR for SARS-CoV-2 detection

Ariel Cerda, Maira Rivera, Grace Armijo, Catalina Ibarra-Henriquez, Javiera Reyes, Paula Blázquez-Sánchez, Javiera Avilés, Aníbal Arce, Aldo Seguel, Alexander J. Brown, Yesseny Vásquez, Marcelo Cortez-San Martín, Francisco A. Cubillos, Patricia García, Marcela Ferres, César A. Ramírez-Sarmiento, Fernán Federici, Rodrigo A. Gutiérrez

published 25 Jan 2024

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Neutrophil extracellular traps in CSF and serum of dogs with steroid-responsive meningitis-arteritis

Jan Christian Wohlsein, Marita Meurer, Matthias Mörgelin, Jasmin Nicole Nessler, Thomas Flegel, Henning Christian Schenk, Konrad Jurina, Kai Rentmeister, Andrea Fischer, Thomas Gödde, Wolfgang Baumgärtner, Maren von Köckritz-Blickwede, Andrea Tipold

published 19 Jan 2024

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Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene

Muhammad Nazmul Hasan, Toshinori Hyodo, Mrityunjoy Biswas, Md. Lutfur Rahman, Yuko Mihara, Sivasundaram Karnan, Akinobu Ota, Shinobu Tsuzuki, Yoshitaka Hosokawa, Hiroyuki Konishi

published 09 Nov 2023

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DNase I targeted degradation of neutrophil extracellular traps to reduce the damage on IgAV rat

Xiu-Qi Chen, Li Tu, Qing Tang, Jia-Sen Zou, Xiang Yun, Yuan-Han Qin

published 31 Oct 2023

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Predicting CRISPR-Cas12a guide efficiency for targeting using machine learning

Aidan O’Brien, Denis C. Bauer, Gaetan Burgio

published 17 Oct 2023

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Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae

Nina Alperovich, Benjamin M. Scott, David Ross

published 17 Oct 2023

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