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Next-generation sequencing

Detection of MYD88 L265P mutation by next-generation deep sequencing in peripheral blood mononuclear cells of Waldenström’s macroglobulinemia and IgM monoclonal gammopathy of undetermined significance

Ayako Nakamura, Chikako Ohwada, Masahiro Takeuchi, Yusuke Takeda, Shokichi Tsukamoto, Naoya Mimura, Oshima-Hasegawa Nagisa, Yasumasa Sugita, Hiroaki Tanaka, Hisashi Wakita, Nobuyuki Aotsuka, Kosei Matsue, Koutaro Yokote, Osamu Ohara, Chiaki Nakaseko, Emiko Sakaida

published 04 Sep 2019

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Bayesian hierarchical negative binomial models for multivariable analyses with applications to human microbiome count data

Amanda H. Pendegraft, Boyi Guo, Nengjun Yi

published 22 Aug 2019

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A research-based gene panel to investigate breast, ovarian and prostate cancer genetic risk

Madison R. Bishop, Anna L. W. Huskey, John Hetzel, Nancy D. Merner

published 15 Aug 2019

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Improvement in detecting cytomegalovirus drug resistance mutations in solid organ transplant recipients with suspected resistance using next generation sequencing

Rubén López-Aladid, Alba Guiu, Maria Mar Mosquera, Francisco López-Medrano, Frederic Cofán, Laura Linares, Julián Torre-Cisneros, Elisa Vidal, Asunción Moreno, Jose María Aguado, Elisa Cordero, Cecilia Martin-Gandul, Jordi Carratalá, Nuria Sabé, Jordi Niubó, Carlos Cervera, Alicia Capón, Anna Cervilla, Marta Santos, Marta Bodro, Patricia Muñoz, Maria Carmen Fariñas, Andrés Antón, Maitane Aranzamendi, Miguel Montejo, Pilar Pérez-Romero, Oscar Len, Maria Ángeles Marcos

published 18 Jul 2019

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Evaluation of KRAS, NRAS and BRAF hotspot mutations detection for patients with metastatic colorectal cancer using direct DNA pipetting in a fully-automated platform and Next-Generation Sequencing for laboratory workflow optimisation

Pauline Gilson, Claire Franczak, Ludovic Dubouis, Marie Husson, Marie Rouyer, Jessica Demange, Marie Perceau, Agnès Leroux, Jean-Louis Merlin, Alexandre Harlé

published 02 Jul 2019

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MicroRNA expression profile in retina and choroid in oxygen-induced retinopathy model

Michel Desjarlais, Jose Carlos Rivera, Isabelle Lahaie, Gaël Cagnone, Maëlle Wirt, Samy Omri, Sylvain Chemtob

published 12 Jun 2019

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Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency

Ignacio García-Tuñón, Verónica Alonso-Pérez, Elena Vuelta, Sandra Pérez- Ramos, María Herrero, Lucía Méndez, Jesús María Hernández-Sánchez, Marta Martín-Izquierdo, Raquel Saldaña, Julián Sevilla, Fermín Sánchez- Guijo, Jesús María Hernández-Rivas, Manuel Sánchez-Martín

published 09 May 2019

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Mining and characterization of novel EST-SSR markers of Parrotia subaequalis (Hamamelidaceae) from the first Illumina-based transcriptome datasets

Yunyan Zhang, Mengyuan Zhang, Yimin Hu, Xin Zhuang, Wuqin Xu, Pengfu Li, Zhongsheng Wang

published 06 May 2019

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Hepatic DNA methylation and expression profiles under prenatal restricted diet in three generations of female rat fetuses

Joanna Nowacka-Woszuk, Adrian Grzemski, Magdalena Sliwinska, Agata Chmurzynska

published 16 Apr 2019

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Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods

Samantha J. McDonough, Aditya Bhagwate, Zhifu Sun, Chen Wang, Michael Zschunke, Joshua A. Gorman, Karla J. Kopp, Julie M. Cunningham

published 11 Apr 2019

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Validation of publicly-available software used in analyzing NGS data for HIV-1 drug resistance mutations and transmission networks in a Washington, DC, Cohort

Kamwing Jair, Chase D. McCann, Harrison Reed, Amanda D. Castel, Marcos Pérez-Losada, Brittany Wilbourn, Alan E. Greenberg, Jeanne A. Jordan, the DC Cohort Executive Committee

published 09 Apr 2019

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Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Konstantin B. Ignatov, Konstantin A. Blagodatskikh, Dmitry S. Shcherbo, Tatiana V. Kramarova, Yulia A. Monakhova, Vladimir M. Kramarov

published 01 Apr 2019

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Baseline identification of clonal V(D)J sequences for DNA-based minimal residual disease detection in multiple myeloma

Even H. Rustad, Malin Hultcrantz, Venkata D. Yellapantula, Theresia Akhlaghi, Caleb Ho, Maria E. Arcila, Mikhail Roshal, Akshar Patel, Denise Chen, Sean M. Devlin, Austin Jacobsen, Ying Huang, Jeffrey E. Miller, Elli Papaemmanuil, Ola Landgren

published 22 Mar 2019

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