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Next-generation sequencing

NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

Ravi K. Patel, Mukesh Jain

Direct Comparisons of Illumina vs. Roche 454 Sequencing Technologies on the Same Microbial Community DNA Sample

Chengwei Luo, Despina Tsementzi, [ ... ], Konstantinos T. Konstantinidis

An Extensive Evaluation of Read Trimming Effects on Illumina NGS Data Analysis

Cristian Del Fabbro, Simone Scalabrin, Michele Morgante, Federico M. Giorgi

A Practical Comparison of De Novo Genome Assembly Software Tools for Next-Generation Sequencing Technologies

Wenyu Zhang, Jiajia Chen, [ ... ], Bairong Shen

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies

Patrick D. Schloss, Dirk Gevers, Sarah L. Westcott

Examining Sources of Error in PCR by Single-Molecule Sequencing

Vladimir Potapov, Jennifer L. Ong

Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNA

Richard B. Lanman, Stefanie A. Mortimer, [ ... ], AmirAli Talasaz

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing

Shunsuke Takahashi, Junko Tomita, [ ... ], Miyuki Nishijima

SNP Calling, Genotype Calling, and Sample Allele Frequency Estimation from New-Generation Sequencing Data

Rasmus Nielsen, Thorfinn Korneliussen, [ ... ], Jun Wang

Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling

Ching Jian, Panu Luukkonen, [ ... ], Katri Korpela

DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

Michele Simbolo, Marisa Gottardi, [ ... ], Aldo Scarpa

Effects of GC Bias in Next-Generation-Sequencing Data on De Novo Genome Assembly

Yen-Chun Chen, Tsunglin Liu, [ ... ], Chi-Chuan Hwang

Estimates of Genetic Differentiation Measured by F_ST Do Not Necessarily Require Large Sample Sizes When Using Many SNP Markers

Eva-Maria Willing, Christine Dreyer, Cock van Oosterhout

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