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Mutagenesis

Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa

Seong-Bin Kim, Salme Timmusk

A Tobacco Etch Virus Protease with Increased Substrate Tolerance at the P1' position

Christian Renicke, Roberta Spadaccini, Christof Taxis

Active-State Models of Ternary GPCR Complexes: Determinants of Selective Receptor-G-Protein Coupling

Ralf C. Kling, Harald Lanig, Timothy Clark, Peter Gmeiner

A TILLING Platform for Functional Genomics in Brachypodium distachyon

Marion Dalmais, Sébastien Antelme, [ ... ], Richard Sibout

Longitudinal Analysis of CCR5 and CXCR4 Usage in a Cohort of Antiretroviral Therapy-Naïve Subjects with Progressive HIV-1 Subtype C Infection

Martin R. Jakobsen, Kieran Cashin, [ ... ], Paul R. Gorry

Engineering of Family-5 Glycoside Hydrolase (Cel5A) from an Uncultured Bacterium for Efficient Hydrolysis of Cellulosic Substrates

Amar A. Telke, Ningning Zhuang, [ ... ], Seon-Won Kim

SOMA: A Single Oligonucleotide Mutagenesis and Cloning Approach

Thorsten Pfirrmann, Ashwin Lokapally, [ ... ], Thomas Hollemann

Substrate Specificity Provides Insights into the Sugar Donor Recognition Mechanism of O-GlcNAc Transferase (OGT)

Xiaofeng Ma, Pi Liu, [ ... ], Lianwen Zhang

In silico Driven Redesign of a Clinically Relevant Antibody for the Treatment of GD2 Positive Tumors

Mahiuddin Ahmed, Yehuda Goldgur, [ ... ], Nai-Kong V. Cheung

The Role of the N-D1 Linker of the N-Ethylmaleimide-Sensitive Factor in the SNARE Disassembly

Cui-Cui Liu, Shan Sun, Sen-Fang Sui

Genotype to Phenotype Mapping and the Fitness Landscape of the E. coli lac Promoter

Jakub Otwinowski, Ilya Nemenman

Quantitative Design of Regulatory Elements Based on High-Precision Strength Prediction Using Artificial Neural Network

Hailin Meng, Jianfeng Wang, [ ... ], Yong Wang

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