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Guide RNA

CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1

Aygul Ishemgulova, Jana Hlaváčová, Karolina Majerová, Anzhelika Butenko, Julius Lukeš, Jan Votýpka, Petr Volf, Vyacheslav Yurchenko

published 13 Feb 2018

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Editing of the human TRIM5 gene to introduce mutations with the potential to inhibit HIV-1

Caroline Dufour, Alix Claudel, Nicolas Joubarne, Natacha Merindol, Tara Maisonnet, Nasser Masroori, Mélodie B. Plourde, Lionel Berthoux

published 26 Jan 2018

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Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1)

Margaret J. Kell, Rachel E. Riccio, Emily A. Baumgartner, Zachary J. Compton, Paul J. Pecorin, Taylor A. Mitchell, Jacek Topczewski, Elizabeth E. LeClair

published 02 Jan 2018

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Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression

Fatwa Adikusuma, Chandran Pfitzner, Paul Quinton Thomas

published 06 Dec 2017

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Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Megan Basila, Melissa L. Kelley, Anja van Brabant Smith

published 27 Nov 2017

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CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe

published 18 Oct 2017

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Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

Thomas W. Kirchner, Markus Niehaus, Thomas Debener, Manfred K. Schenk, Marco Herde

published 22 Sep 2017

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Cas9/gRNA targeted excision of cystic fibrosis-causing deep-intronic splicing mutations restores normal splicing of CFTR mRNA

David J. Sanz, Jennifer A. Hollywood, Martina F. Scallan, Patrick T. Harrison

published 01 Sep 2017

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Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Olaf Voets, Frans Tielen, Edo Elstak, Julian Benschop, Max Grimbergen, Jan Stallen, Richard Janssen, Andre van Marle, Christian Essrich

published 11 Aug 2017

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Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish

Yunru Chen, Shiyang Zeng, Ruikun Hu, Xiangxiu Wang, Weilai Huang, Jiangfang Liu, Luying Wang, Guifen Liu, Ying Cao, Yong Zhang

published 11 Aug 2017

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IFNAR2-dependent gene expression profile induced by IFN-α in Pteropus alecto bat cells and impact of IFNAR2 knockout on virus infection

Qian Zhang, Lei-Ping Zeng, Peng Zhou, Aaron T. Irving, Shang Li, Zheng-Li Shi, Lin-Fa Wang

published 09 Aug 2017

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HEK293T cell lines defective for O-linked glycosylation

James M. Termini, Zachary A. Silver, Bryony Connor, Aristotelis Antonopoulos, Stuart M. Haslam, Anne Dell, Ronald C. Desrosiers

published 27 Jun 2017

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Plasmid-free CRISPR/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733

Emily D. Crawford, Jenai Quan, Jeremy A. Horst, Daniel Ebert, Wesley Wu, Joseph L. DeRisi

published 22 May 2017

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