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Guide RNA

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

Letian Song, Jean-Paul Ouedraogo, [ ... ], Adrian Tsang

A G542X cystic fibrosis mouse model for examining nonsense mutation directed therapies

Daniel R. McHugh, Miarasa S. Steele, [ ... ], Craig A. Hodges

CRISPR/Cas-based customization of pooled CRISPR libraries

Jiyeon Kweon, Da-eun Kim, An-Hee Jang, Yongsub Kim

A CRISPR screen identifies MAPK7 as a target for combination with MEK inhibition in KRAS mutant NSCLC

Nicholas Dompe, Christiaan Klijn, [ ... ], David Stokoe

MAL73, a novel regulator of maltose fermentation, is functionally impaired by single nucleotide polymorphism in sake brewing yeast

Takumi Ohdate, Fumihiko Omura, [ ... ], Eiichiro Ono

DMSO increases efficiency of genome editing at two non-coding loci

George Stratigopoulos, Maria Caterina De Rosa, [ ... ], Claudia A. Doege

CRISPR/Cas9 mediated high efficiency knockout of the eye color gene Vermillion in Helicoverpa zea (Boddie)

Omaththage P. Perera, Nathan S. Little, Calvin A. Pierce III

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

Simon E. Tröder, Lena K. Ebert, [ ... ], Branko Zevnik

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Christopher T. Breunig, Tamara Durovic, [ ... ], Stefan H. Stricker

Expanding the genetic toolkit of Tribolium castaneum

Johnathan C. Rylee, Dylan J. Siniard, [ ... ], Andrew C. Zelhof

Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators

Henriette O’Geen, Chonghua Ren, [ ... ], David J. Segal

Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9

Kazuhisa Honma, Satoshi Abe, [ ... ], Yasuhiro Kazuki

Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene

Janina Gergen, Flora Coulon, [ ... ], Fabienne Haspot

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