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Guide RNA

A G542X cystic fibrosis mouse model for examining nonsense mutation directed therapies

Daniel R. McHugh, Miarasa S. Steele, Dana M. Valerio, Alexander Miron, Rachel J. Mann, David F. LePage, Ronald A. Conlon, Calvin U. Cotton, Mitchell L. Drumm, Craig A. Hodges

published 20 Jun 2018

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CRISPR/Cas-based customization of pooled CRISPR libraries

Jiyeon Kweon, Da-eun Kim, An-Hee Jang, Yongsub Kim

published 20 Jun 2018

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A CRISPR screen identifies MAPK7 as a target for combination with MEK inhibition in KRAS mutant NSCLC

Nicholas Dompe, Christiaan Klijn, Sara A. Watson, Katherine Leng, Jenna Port, Trinna Cuellar, Colin Watanabe, Benjamin Haley, Richard Neve, Marie Evangelista, David Stokoe

published 18 Jun 2018

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MAL73, a novel regulator of maltose fermentation, is functionally impaired by single nucleotide polymorphism in sake brewing yeast

Takumi Ohdate, Fumihiko Omura, Haruyo Hatanaka, Yan Zhou, Masami Takagi, Tetsuya Goshima, Takeshi Akao, Eiichiro Ono

published 12 Jun 2018

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DMSO increases efficiency of genome editing at two non-coding loci

George Stratigopoulos, Maria Caterina De Rosa, Charles A. LeDuc, Rudolph L. Leibel, Claudia A. Doege

published 04 Jun 2018

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CRISPR/Cas9 mediated high efficiency knockout of the eye color gene Vermillion in Helicoverpa zea (Boddie)

Omaththage P. Perera, Nathan S. Little, Calvin A. Pierce III

published 17 May 2018

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An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

Simon E. Tröder, Lena K. Ebert, Linus Butt, Sonja Assenmacher, Bernhard Schermer, Branko Zevnik

published 03 May 2018

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One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Christopher T. Breunig, Tamara Durovic, Andrea M. Neuner, Valentin Baumann, Maximilian F. Wiesbeck, Anna Köferle, Magdalena Götz, Jovica Ninkovic, Stefan H. Stricker

published 27 Apr 2018

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Expanding the genetic toolkit of Tribolium castaneum

Johnathan C. Rylee, Dylan J. Siniard, Kaitlin Doucette, Gabriel E. Zentner, Andrew C. Zelhof

published 12 Apr 2018

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Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators

Henriette O’Geen, Chonghua Ren, Nicole B. Coggins, Sofie L. Bates, David J. Segal

published 27 Mar 2018

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Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9

Kazuhisa Honma, Satoshi Abe, Takeshi Endo, Narumi Uno, Mitsuo Oshimura, Tetsuya Ohbayashi, Yasuhiro Kazuki

published 05 Mar 2018

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Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene

Janina Gergen, Flora Coulon, Alison Creneguy, Nathan Elain-Duret, Alejandra Gutierrez, Olaf Pinkenburg, Els Verhoeyen, Ignacio Anegon, Tuan Huy Nguyen, Franck Albert Halary, Fabienne Haspot

published 15 Feb 2018

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CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1

Aygul Ishemgulova, Jana Hlaváčová, Karolina Majerová, Anzhelika Butenko, Julius Lukeš, Jan Votýpka, Petr Volf, Vyacheslav Yurchenko

published 13 Feb 2018

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