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Guide RNA

Constructing a human complex type N-linked glycosylation pathway in Kluyveromyces marxianus

Ming-Hsuan Lee, Tsui-Ling Hsu, Jinn-Jy Lin, Yu-Ju Lin, Yi-Ying Kao, Jui-Jen Chang, Wen-Hsiung Li

published 29 May 2020

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TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

Tyler Picariello, Yuqing Hou, Tomohiro Kubo, Nathan A. McNeill, Haru-aki Yanagisawa, Toshiyuki Oda, George B. Witman

published 13 May 2020

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SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Derek Cerchione, Katherine Loveluck, Eric L. Tillotson, Fred Harbinski, Jen DaSilva, Chase P. Kelley, Elise Keston-Smith, Cecilia A. Fernandez, Vic E. Myer, Hariharan Jayaram, Barrett E. Steinberg

published 16 Apr 2020

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CRISPR/Cas9-targeting of CD40 in hematopoietic stem cells limits immune activation mediated by anti-CD40

Rui Wang, Sean Graham, Ning Sun, Donna McCarthy, Ruoqi Peng, Jamie Erickson, Liz Oconnor, Xiaochun Zhu, Marc Wurbel, Robert Dunstan, Susan Westmoreland, Namjin Chung, Tariq Ghayur, Jijie Gu

published 10 Mar 2020

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CRISPR-mediated accelerated domestication of African rice landraces

Elia Lacchini, Edward Kiegle, Marco Castellani, Hélène Adam, Stefan Jouannic, Veronica Gregis, Martin M. Kater

published 03 Mar 2020

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An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, Longfei Huo, Lang Ma, Ailing W. Scott, Melissa Pool Pizzi, Yuan Li, Ying Wang, Xiaodan Yao, Shumei Song, Jaffer A. Ajani

published 13 Feb 2020

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Are the current gRNA ranking prediction algorithms useful for genome editing in plants?

Fatima Naim, Kylie Shand, Satomi Hayashi, Martin O’Brien, James McGree, Alexander A. T. Johnson, Benjamin Dugdale, Peter M. Waterhouse

published 24 Jan 2020

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Tankyrase inhibition sensitizes cells to CDK4 blockade

Miguel Foronda, Yusuke Tarumoto, Emma M. Schatoff, Benjamin I. Leach, Bianca J. Diaz, Jill Zimmerman, Sukanya Goswami, Michael Shusterman, Christopher R. Vakoc, Lukas E. Dow

published 31 Dec 2019

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High throughput, efficacious gene editing & genome surveillance in Chinese hamster ovary cells

S. C. Huhn, Y. Ou, A. Kumar, R. Liu, Z. Du

published 19 Dec 2019

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Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development

Anirban Roy, Ying Zhai, Jessica Ortiz, Michael Neff, Bikash Mandal, Sunil Kumar Mukherjee, Hanu R. Pappu

published 23 Oct 2019

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Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient

Sophie Dal-Pra, Conrad P. Hodgkinson, Victor J. Dzau

published 17 Oct 2019

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CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein

Milad Babaei, Yuekun Liu, Shelly M. Wuerzberger-Davis, Ethan Z. McCaslin, Christopher J. DiRusso, Alan T. Yeo, Larisa Kagermazova, Shigeki Miyamoto, Thomas D. Gilmore

published 25 Sep 2019

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Specific inter-domain interactions stabilize a compact HIV-1 Gag conformation

Chen Lin, Paola Mendoza-Espinosa, Ioulia Rouzina, Orlando Guzmán, José Antonio Moreno-Razo, Joseph S. Francisco, Robijn Bruinsma

published 22 Aug 2019

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