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Guide RNA

Lung development, repair and cancer: A study on the role of MMP20 gene in adenocarcinoma

Pooi Ling Mok, Arun Neela Kumar Anandasayanam, Hernandez Maradiaga Oscar David, Jiabei Tong, Aisha Farhana, Mohammed Safwan Ali Khan, Gothai Sivaprakasam, Avin Ee-Hwan Koh, Badr Alzahrani

published 29 Apr 2021

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CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing

Amelia D. Wallace, Thomas A. Sasani, Jordan Swanier, Brooke L. Gates, Jeff Greenland, Brent S. Pedersen, Katherine E. Varley, Aaron R. Quinlan

published 08 Apr 2021

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Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning

Tobias Gross, Csaba Jeney, Darius Halm, Günter Finkenzeller, G. Björn Stark, Roland Zengerle, Peter Koltay, Stefan Zimmermann

published 04 Mar 2021

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An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes

Cristina Leoni, Niccolò Bianchi, Lucia Vincenzetti, Silvia Monticelli

published 18 Feb 2021

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Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila melanogaster

Cristin Chon, Grace Chon, Yurika Matsui, Huiqing Zeng, Zhi-Chun Lai, Aimin Liu

published 14 Jan 2021

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Enhancer polymorphisms at the IKZF1 susceptibility locus for acute lymphoblastic leukemia impact B-cell proliferation and differentiation in both Down syndrome and non-Down syndrome genetic backgrounds

Vincent U. Gant, Jacob J. Junco, Maci Terrell, Raushan Rashid, Karen R. Rabin

published 07 Jan 2021

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CRISPR/Cas9-loaded stealth liposomes effectively cleared established HPV16-driven tumours in syngeneic mice

Luqman Jubair, Alfred K. Lam, Sora Fallaha, Nigel A. J. McMillan

published 07 Jan 2021

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Site-specific targeting of a light activated dCas9-KillerRed fusion protein generates transient, localized regions of oxidative DNA damage

Nealia C. M. House, Ramya Parasuram, Jacob V. Layer, Brendan D. Price

published 17 Dec 2020

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Ares-GT: Design of guide RNAs targeting multiple genes for CRISPR-Cas experiments

Eugenio Gómez Minguet

published 21 Oct 2020

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Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay

Pin Lyu, Kyung Whan Yoo, Manish Kumar Yadav, Anthony Atala, Annemieke Aartsma-Rus, Maaike van Putten, Dongsheng Duan, Baisong Lu

published 24 Sep 2020

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Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

Louhanna Pinheiro Rodrigues Teixeira, Francisco Eder de Moura Lopes, André Saraiva Leão Marcelo Antunes, Matheus Soares Alves, André Marrocos Miranda, Saul Gaudencio Neto, Leonardo Tondello Martins, Ana Cristina de Oliveira Monteiro Moreira, Kaio Cesar Simiano Tavares

published 18 Sep 2020

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Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site

Stuart J. Smith, Norma Towers, Kim Demetriou, Timothy J. Mohun

published 29 Jul 2020

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Super-enhancer acquisition drives oncogene expression in triple negative breast cancer

Ryan Raisner, Russell Bainer, Peter M. Haverty, Kelli L. Benedetti, Karen E. Gascoigne

published 25 Jun 2020

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