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Guide RNA

Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems

Julia K. Bialek, Gábor A. Dunay, [ ... ], Ulrike C. Lange

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Megan Basila, Melissa L. Kelley, Anja van Brabant Smith

Cas9/gRNA targeted excision of cystic fibrosis-causing deep-intronic splicing mutations restores normal splicing of CFTR mRNA

David J. Sanz, Jennifer A. Hollywood, Martina F. Scallan, Patrick T. Harrison

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells

Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin

Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells

Olaf Voets, Frans Tielen, [ ... ], Christian Essrich

A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko, Joseph Giacalone, [ ... ], Willie H. Mark

Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

Louhanna Pinheiro Rodrigues Teixeira, Francisco Eder de Moura Lopes, [ ... ], Kaio Cesar Simiano Tavares

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li, Saurabh Mendiratta, [ ... ], Leonidas Bleris

DMSO increases efficiency of genome editing at two non-coding loci

George Stratigopoulos, Maria Caterina De Rosa, [ ... ], Claudia A. Doege

TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

Tyler Picariello, Yuqing Hou, [ ... ], George B. Witman

Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata

Thomas W. Kirchner, Markus Niehaus, [ ... ], Marco Herde

CRISPR-mediated accelerated domestication of African rice landraces

Elia Lacchini, Edward Kiegle, [ ... ], Martin M. Kater

Plasmid-free CRISPR/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733

Emily D. Crawford, Jenai Quan, [ ... ], Joseph L. DeRisi

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