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Guide RNA

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

Lazar Dimitrov, Darlene Pedersen, [ ... ], Philip A. Leighton

Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3´-Translational Enhancer that Communicates with the Corresponding 5´-Region through a Long-Distance RNA-RNA Interaction

Marta Blanco-Pérez, Miryam Pérez-Cañamás, Leticia Ruiz, Carmen Hernández

Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System

Gary Alan Barclay Armstrong, Meijiang Liao, [ ... ], Pierre Drapeau

A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko, Joseph Giacalone, [ ... ], Willie H. Mark

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li, Saurabh Mendiratta, [ ... ], Leonidas Bleris

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo

Minyan Li, Rui Huang, [ ... ], Junjiu Huang

Simple Genome Editing of Rodent Intact Embryos by Electroporation

Takehito Kaneko, Tomoji Mashimo

CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection

Hassen S. Wollebo, Anna Bellizzi, [ ... ], Kamel Khalili

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

Maxim Kostylev, Anne E. Otwell, Ruth E. Richardson, Yo Suzuki

The Evolution of the Secreted Regulatory Protein Progranulin

Roger G. E. Palfree, Hugh P. J. Bennett, Andrew Bateman

Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering

Mustapha Aouida, Ayman Eid, [ ... ], Magdy Mahfouz

Inactivation of ca10a and ca10b Genes Leads to Abnormal Embryonic Development and Alters Movement Pattern in Zebrafish

Ashok Aspatwar, Martti E. E. Tolvanen, [ ... ], Seppo Parkkila

CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells

Misako Haraguchi, Masahiro Sato, Masayuki Ozawa

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