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Guide RNA

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

Lazar Dimitrov, Darlene Pedersen, Kathryn H. Ching, Henry Yi, Ellen J. Collarini, Shelley Izquierdo, Marie-Cecile van de Lavoir, Philip A. Leighton

published 21 Apr 2016

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Efficient Translation of Pelargonium line pattern virus RNAs Relies on a TED-Like 3´-Translational Enhancer that Communicates with the Corresponding 5´-Region through a Long-Distance RNA-RNA Interaction

Marta Blanco-Pérez, Miryam Pérez-Cañamás, Leticia Ruiz, Carmen Hernández

published 04 Apr 2016

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Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System

Gary Alan Barclay Armstrong, Meijiang Liao, Zhipeng You, Alexandra Lissouba, Brian Edwin Chen, Pierre Drapeau

published 01 Mar 2016

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A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

Peter J. Romanienko, Joseph Giacalone, Joanne Ingenito, Yijie Wang, Mayumi Isaka, Thomas Johnson, Yun You, Willie H. Mark

published 05 Feb 2016

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Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

Yi Li, Saurabh Mendiratta, Kristina Ehrhardt, Neha Kashyap, Michael A. White, Leonidas Bleris

published 20 Jan 2016

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CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo

Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan, Shanbo Cao, Zhou Songyang, Junjiu Huang

published 24 Nov 2015

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Simple Genome Editing of Rodent Intact Embryos by Electroporation

Takehito Kaneko, Tomoji Mashimo

published 10 Nov 2015

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CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection

Hassen S. Wollebo, Anna Bellizzi, Rafal Kaminski, Wenhui Hu, Martyn K. White, Kamel Khalili

published 11 Sep 2015

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Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

Maxim Kostylev, Anne E. Otwell, Ruth E. Richardson, Yo Suzuki

published 08 Sep 2015

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The Evolution of the Secreted Regulatory Protein Progranulin

Roger G. E. Palfree, Hugh P. J. Bennett, Andrew Bateman

published 06 Aug 2015

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Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering

Mustapha Aouida, Ayman Eid, Zahir Ali, Thomas Cradick, Ciaran Lee, Harshavardhan Deshmukh, Ahmed Atef, Dina AbuSamra, Samah Zeineb Gadhoum, Jasmeen Merzaban, Gang Bao, Magdy Mahfouz

published 30 Jul 2015

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Inactivation of ca10a and ca10b Genes Leads to Abnormal Embryonic Development and Alters Movement Pattern in Zebrafish

Ashok Aspatwar, Martti E. E. Tolvanen, Markus J. T. Ojanen, Harlan R. Barker, Anni K. Saralahti, Carina A. Bäuerlein, Csaba Ortutay, Peiwen Pan, Marianne Kuuslahti, Mataleena Parikka, Mika Rämet, Seppo Parkkila

published 28 Jul 2015

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CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells

Misako Haraguchi, Masahiro Sato, Masayuki Ozawa

published 10 Jul 2015

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