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Guide RNA

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

Maxim Kostylev, Anne E. Otwell, Ruth E. Richardson, Yo Suzuki

Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Kai Li, Gang Wang, [ ... ], William T. Pu

A system for site-specific integration of transgenes in mammalian cells

Xiuling Chi, Qi Zheng, [ ... ], Ling-Jie Kong

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System

Wei Ni, Jun Qiao, [ ... ], Chuangfu Chen

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, [ ... ], Jaffer A. Ajani

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

Shigeo S. Sugano, Ryuichi Nishihama, [ ... ], Takayuki Kohchi

Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation

Rolf B. Edvardsen, Sven Leininger, [ ... ], Anna Wargelius

CRISPR-cas gene-editing as plausible treatment of neuromuscular and nucleotide-repeat-expansion diseases: A systematic review

Haris Babačić, Aditi Mehta, Olivia Merkel, Benedikt Schoser

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

Morito Kurata, Natalie K. Wolf, [ ... ], Branden S. Moriarity

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ Cells

Lazar Dimitrov, Darlene Pedersen, [ ... ], Philip A. Leighton

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

Letian Song, Jean-Paul Ouedraogo, [ ... ], Adrian Tsang

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

Simon E. Tröder, Lena K. Ebert, [ ... ], Branko Zevnik

Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity Purification

Nicholas J. Matheson, Andrew A. Peden, Paul J. Lehner

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