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Gene targeting

Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system

Katsuyuki Nakamura, Sara Ito, Yoshihiro Ohguchi, Toshie Jimbo, Yusaku Wada, Ryota Nakajima, Masanobu Kanou, Kei Yamana, Hiroshi Ueda

published 25 Jun 2025

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Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries

Wajdi Ayadi, Fahmi Smaoui, Saba Gargouri, Sana Ferjani, Zaineb Hamzaoui, Awatef Taktak, Amel Chtourou, Houda Skouri-Gargouri, Azza Hadj Sassi, Mouna Ben Sassi, Sameh Trabelsi, Ali Gargouri, Ilhem Boutiba-Ben Boubaker, Héla Karray-Hakim, Raja Mokdad-Gargouri, Lamia Feki-Berrajah

published 05 May 2025

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Development of a lambda Red based system for gene deletion in Chlamydia

Yibing Wang, Robert Suchland, Amy Hua, Steven Carrell, Daniel Rockey, Kevin Hybiske

published 14 Nov 2024

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Non-replicative phage particles delivering CRISPR-Cas9 to target major bla_CTX-M variants

Naiyaphat Nittayasut, Teerapong Yata, Sunisa Chirakul, Navapon Techakriengkrai, Pattrarat Chanchaithong

published 16 May 2024

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Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach

Umi Nuraeni, Jekmal Malau, Retno Tri Astuti, Auraga Dewantoro, Dini Apriori, Evellin Dewi Lusiana, Bambang Prasetya

published 14 Jul 2023

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Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

published 24 Feb 2023

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Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

published 20 Jan 2023

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Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4

Shuang Li, Yichao Tang, Lingran Tang, Xuanyu Yan, Jiali Xiao, Huijun Xiang, Qing Wu, Ruqi Yu, Yushi Jin, Jingyu Yu, Nuo Xu, Chu Wu, Shengqin Wang, Chuanhua Wang, Qiongzhen Chen

published 02 Dec 2022

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The deletion of HK-1 gene affects the bacterial virulence of Pseudomonas stutzeri LH-42

Si Shan, Tingting Hu, Yu Yang

published 29 Nov 2022

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High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

published 09 Sep 2022

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Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray

Tate Oulton, Joshua Obiero, Isabel Rodriguez, Isaac Ssewanyana, Rebecca A. Dabbs, Christine M. Bachman, Bryan Greenhouse, Chris Drakeley, Phil L. Felgner, Will Stone, Kevin K. A. Tetteh

published 29 Aug 2022

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Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum

Nahyun Lee, Jiyeun Park, Jung-Eun Kim, Ji Young Shin, Kyunghun Min, Hokyoung Son

published 03 Jun 2022

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Identification of miRNA signature for predicting the prognostic biomarker of squamous cell lung carcinoma

Huanqing Liu, Tingting Li, Chunsheng Dong, Jun Lyu

published 15 Mar 2022

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