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Gene cloning

Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene

Daria Krefft, Aliaksei Papkov, Agnieszka Zylicz-Stachula, Piotr M. Skowron

Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells

Shinsaku Tokuda, Toyohiro Hirai, Mikio Furuse

Metabolic engineering of CHO cells for the development of a robust protein production platform

Sanjeev Kumar Gupta, Santosh K. Srivastava, [ ... ], Pratyoosh Shukla

HSF1 and HSF3 cooperatively regulate the heat shock response in lizards

Ryosuke Takii, Mitsuaki Fujimoto, [ ... ], Akira Nakai

Molecular and microbiological report of a hospital outbreak of NDM-1-carrying Enterobacteriaceae in Mexico

Paola Bocanegra-Ibarias, Elvira Garza-González, [ ... ], Jesus Silva-Sanchez

Genomic and phenotypic characterisation of fluoroquinolone resistance mechanisms in Enterobacteriaceae in Durban, South Africa

John Osei Sekyere, Daniel Gyamfi Amoako

Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research

Mostafa Aboulela, Yuji Tanaka, [ ... ], Tsuyoshi Nakagawa

Correction of PTEN mutations in glioblastoma cell lines via AAV-mediated gene editing

Victoria K. Hill, Jung-Sik Kim, C. David James, Todd Waldman

QTL analysis and dissection of panicle components in rice using advanced backcross populations derived from Oryza Sativa cultivars HR1128 and ‘Nipponbare’

Zhizhong Sun, Xiaoling Yin, [ ... ], Jun Fang

Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens

Noga Yaakov, Yoav Barak, [ ... ], Michael Elbaum

Variation in gene expression within clones of the earthworm Dendrobaena octaedra

Marina Mustonen, Jari Haimi, [ ... ], K. Emily Knott

LuxCDE-luxAB-based promoter reporter system to monitor the Yersinia enterocolitica O:3 gene expression in vivo

Elif Bozcal, Melih Dagdeviren, Atac Uzel, Mikael Skurnik

Derivative of Extremophilic 50S Ribosomal Protein L35Ae as an Alternative Protein Scaffold

Anna V. Lomonosova, Andrei B. Ulitin, [ ... ], Sergei E. Permyakov

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