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Enzyme purification

Enzymatic properties of a L-asparaginase without secondary glutaminase activity from Streptomyces scabrisporus

Ricardo Rodríguez-Vargas, Francisca Villanueva-Flores, María Fernanda Gutiérrez-Chávez, Carlos Medrano-Villagómez, Andrés Zárate-Romero, Alejandro Huerta-Saquero

published 07 Nov 2025

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Enzymatic characterization and polyurethane biodegradation assay of two novel esterases isolated from a polluted river

Arianna Soto-Hernández, Luis Felipe Muriel-Millán, Adolfo Gracia, Alejandro Sánchez-Flores, Liliana Pardo-López

published 23 Jul 2025

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A test method for selecting suitable cleaning indicators for routine cleaning monitoring on a washer-disinfector in a central sterile supply department

Juan Zhou, Wei Guo, Dongling Liu, Jianrong Li, Caixia Yang, Ying Wang, Xiaoyi Huang

published 01 Jul 2025

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The transformation pathways and optimization of conditions for preparation minor ginsenosides from Panax notoginseng root by the fungus Aspergillus tubingensis

Fei-Xing Li, Dong-Mei Lin, Jin Yang, Xiu-Ming Cui, Xiao-Yan Yang

published 03 Mar 2025

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An Open One-Step RT-qPCR for SARS-CoV-2 detection

Ariel Cerda, Maira Rivera, Grace Armijo, Catalina Ibarra-Henriquez, Javiera Reyes, Paula Blázquez-Sánchez, Javiera Avilés, Aníbal Arce, Aldo Seguel, Alexander J. Brown, Yesseny Vásquez, Marcelo Cortez-San Martín, Francisco A. Cubillos, Patricia García, Marcela Ferres, César A. Ramírez-Sarmiento, Fernán Federici, Rodrigo A. Gutiérrez

published 25 Jan 2024

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Characterization and applications of glutaminase free L-asparaginase from indigenous Bacillus halotolerans ASN9

Ifrah Shafqat, Shaheen Shahzad, Azra Yasmin, Muhammad Tausif Chaudhry, Safia Ahmed, Aneela Javed, Imran Afzal, Monaza Bibi

published 28 Nov 2023

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Purification and characterization of novel isoforms of the polyphenol oxidase from Malus domestica fruit pulp

Naila Sajjad, M. Sheeraz Ahmad, Raja Tahir Mahmood, Muhammad Tariq, Muhammad Javaid Asad, Shamaila Irum, Anisa Andleeb, Abid Riaz, Dawood Ahmed

published 25 Aug 2023

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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris

William Donelan, ShiWu Li, Paul R. Dominguez-Gutierrez, Augustus Anderson IV, Li-Jun Yang, Cuong Nguyen, Benjamin K. Canales

published 11 May 2023

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Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya

Hina Mushtaq, Shabir Ahmad Ganai, Arshid Jehangir, Bashir Ahmad Ganai, Rubiya Dar

published 30 Mar 2023

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Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11

Muhammad Saqib Malik, Abdul Rehman, Irfan Ullah Khan, Taj Ali Khan, Muhammad Jamil, Eui Shik Rha, Muhammad Anees

published 27 Jan 2023

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Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP

Diana Angélica Tapia-Sidas, Brenda Yazmín Vargas-Hernández, José Abrahán Ramírez-Pool, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Rogelio González-González, Luis Gabriel Brieba, Rosalía Lira-Carmona, Eduardo Ferat-Osorio, Constantino López-Macías, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cázares

published 26 Jan 2023

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ATP hydrolytic activity of purified Spf1p correlate with micellar lipid fluidity and is dependent on conserved residues in transmembrane helix M1

Johan Ørskov Ipsen, Danny Mollerup Sørensen

published 20 Oct 2022

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Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein

Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque

published 01 Apr 2022

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