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DNA manipulations

A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species

Robert J. Elshire, Jeffrey C. Glaubitz, [ ... ], Sharon E. Mitchell

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

Evaluation of Methods for the Extraction and Purification of DNA from the Human Microbiome

Sanqing Yuan, Dora B. Cohen, [ ... ], Larry J. Forney

Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

Pamela D. Schoppee Bortz, Brian R. Wamhoff

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

Ellen Knierim, Barbara Lucke, [ ... ], Dominik Seelow

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

Swee Jin Tan, Huan Phan, [ ... ], William F. Burkholder

Unlocking Short Read Sequencing for Metagenomics

Sébastien Rodrigue, Arne C. Materna, [ ... ], Sallie W. Chisholm

A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA

George R. Feehery, Erbay Yigit, [ ... ], Sriharsa Pradhan

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

John M. Pryor, Vladimir Potapov, [ ... ], Gregory J. S. Lohman

A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

Theodore L. Roth, Ljiljana Milenkovic, Matthew P. Scott

Library Preparation and Multiplex Capture for Massive Parallel Sequencing Applications Made Efficient and Easy

Mårten Neiman, Simon Sundling, [ ... ], Daniel Klevebring

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning

Eszter Tóth, Krisztina Huszár, [ ... ], Ervin Welker

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