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DNA extraction

AirDNA sampler: An efficient and simple device enabling high-yield, high-quality airborne environment DNA for metagenomic applications

Piyanun Harnpicharnchai, Panyapon Pumkaeo, Paopit Siriarchawatana, Somsak Likhitrattanapisal, Sermsiri Mayteeworakoon, Lily Ingsrisawang, Worawongsin Boonsin, Lily Eurwilaichitr, Supawadee Ingsriswang

published 29 Jun 2023

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On-site detection system of Candidatus Liberibacter asiaticus by using TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR)

Shun-Min Yao, Meng-Ling Wu, Ting-Hsuan Hung

published 23 Jun 2023

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A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong

Vicky Ooi, Lee McMichael, Margaret E. Hunter, Aristide Takoukam Kamla, Janet M. Lanyon

published 07 Jun 2023

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Development of diagnostic and point of care assays for a gammaherpesvirus infecting koalas

Belinda R. Wright, Martina Jelocnik, Andrea Casteriano, Yasmine S. S. Muir, Alistair R. Legione, Paola K. Vaz, Joanne M. Devlin, Damien P. Higgins

published 01 Jun 2023

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A quantitative PCR assay for the detection and quantification of Septoria pistaciarum, the causal agent of pistachio leaf spot in Italy

Mounira Inas Drais, Giorgio Gusella, Angelo Mazzaglia, Giancarlo Polizzi

published 19 May 2023

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Isolation and identification of hyaluronan-degrading bacteria from Japanese fecal microbiota

Hazuki Akazawa, Itsuko Fukuda, Haruna Kaneda, Shoichi Yoda, Mamoru Kimura, Ryohei Nomoto, Shuji Ueda, Yasuhito Shirai, Ro Osawa

published 17 May 2023

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Signal and noise in metabarcoding data

Zachary Gold, Andrew Olaf Shelton, Helen R. Casendino, Joe Duprey, Ramón Gallego, Amy Van Cise, Mary Fisher, Alexander J. Jensen, Erin D’Agnese, Elizabeth Andruszkiewicz Allan, Ana Ramón-Laca, Maya Garber-Yonts, Michaela Labare, Kim M. Parsons, Ryan P. Kelly

published 11 May 2023

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Evaluation of alcohol-free mouthwash for studies of the oral microbiome

Yukiko Yano, Emily Vogtmann, Alaina H. Shreves, Stephanie J. Weinstein, Amanda Black, Norma Diaz-Mayoral, Yunhu Wan, Weiyin Zhou, Xing Hua, Casey L. Dagnall, Amy Hutchinson, Kristine Jones, Belynda D. Hicks, Kathleen Wyatt, Michelle Brotzman, Nicole Gerlanc, Wen-Yi Huang, Paul S. Albert, Nicolas Wentzensen, Christian C. Abnet

published 27 Apr 2023

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Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM)

Antoine Champie, Amélie De Grandmaison, Simon Jeanneau, Frédéric Grenier, Pierre-Étienne Jacques, Sébastien Rodrigue

published 11 Apr 2023

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Quantification of diversity sampling bias resulting from rice root bacterial isolation on popular and nitrogen-free culture media using 16S amplicon barcoding

Moussa Sondo, Issa Wonni, Agnieszka Klonowska, Kadidia Koïta, Lionel Moulin

published 06 Apr 2023

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Multi-faceted metagenomic analysis of spacecraft associated surfaces reveal planetary protection relevant microbial composition

Sarah K. Highlander, Jason M. Wood, John D. Gillece, Megan Folkerts, Viacheslav Fofanov, Tara Furstenau, Nitin K. Singh, Lisa Guan, Arman Seuylemezian, James N. Benardini, David M. Engelthaler, Kasthuri Venkateswaran, Paul S. Keim

published 22 Mar 2023

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A high-throughput newborn screening approach for SCID, SMA, and SCD combining multiplex qPCR and tandem mass spectrometry

Rafael Tesorero, Joachim Janda, Friederike Hörster, Patrik Feyh, Ulrike Mütze, Jana Hauke, Kathrin Schwarz, Joachim B. Kunz, Georg F. Hoffmann, Jürgen G. Okun

published 10 Mar 2023

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Detection of azole-resistant Aspergillus fumigatus in the environment from air, plant debris, compost, and soil

Luisa F. Gómez Londoño, Marin T. Brewer

published 03 Mar 2023

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