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DNA denaturation

A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplification

Ryoyo Ikebuchi, Lu Xi, Dimitra Bouri, Valeriia Svintytska, Hanna Davies, Pawel Olszewski, Bozena Bruhn-Olszewska, Jan P. Dumanski, Ulf Landegren

published 28 Oct 2025

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Enhanced thermal stability enables human mismatch-specific thymine–DNA glycosylase to catalyse futile DNA repair

Diana Manapkyzy, Botagoz Joldybayeva, Alexander A. Ishchenko, Bakhyt T. Matkarimov, Dmitry O. Zharkov, Sabira Taipakova, Murat K. Saparbaev

published 18 Oct 2024

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May DNA analyses be biased by hidden oxidative damage? Voltammetric study of temperature and oxidation stress effect

Oskar Szczepaniak, Marta Ligaj

published 14 Jun 2024

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Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure

Anne R. Shim, Jane Frederick, Emily M. Pujadas, Tiffany Kuo, I. Chae Ye, Joshua A. Pritchard, Cody L. Dunton, Paola Carrillo Gonzalez, Nicolas Acosta, Surbhi Jain, Nicholas M. Anthony, Luay M. Almassalha, Igal Szleifer, Vadim Backman

published 28 May 2024

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Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of α⁰-thalassemia--^SEA,--^THAI, and--^CR type deletions

Chedtapak Ruengdit, Manoo Punyamung, Nutjeera Intasai, Sakorn Pornprasert

published 06 Nov 2023

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Neural network execution using nicked DNA and microfluidics

Arnav Solanki, Zak Griffin, Purab Ranjan Sutradhar, Karisha Pradhan, Caiden Merritt, Amlan Ganguly, Marc Riedel

published 19 Oct 2023

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A qPCR-duplex assay for sex determination in ancient DNA

Anna Poma, Patrizia Cesare, Antonella Bonfigli, Anna Rita Volpe, Sabrina Colafarina, Giulia Vecchiotti, Alfonso Forgione, Osvaldo Zarivi

published 10 Jun 2022

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Interaction between serum levels of Anti-Mullerian Hormone and the degree of sperm DNA fragmentation measured by sperm chromatin structure assay can be a predictor for the outcome of standard in vitro fertilization

Peter Zarén, Sara Alson, Emir Henic, Mona Bungum, Aleksander Giwercman

published 08 Aug 2019

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Tyrosines involved in the activity of φ29 single-stranded DNA binding protein

Iván de la Torre, Victor Quiñones, Margarita Salas, Alicia del Prado

published 20 May 2019

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(C₂G₄)_n repeat expansion sequences from the C9orf72 gene form an unusual DNA higher-order structure in the pH range of 5-6

Prince Kumar Lat, Dipankar Sen

published 18 Jun 2018

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Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekinge

published 14 Jun 2018

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High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous atmosphere

Anne-Lise Fabre, Aurélie Luis, Marthe Colotte, Sophie Tuffet, Jacques Bonnet

published 30 Nov 2017

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In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag

Valeria Visone, Wenyuan Han, Giuseppe Perugino, Giovanni del Monaco, Qunxin She, Mosè Rossi, Anna Valenti, Maria Ciaramella

published 03 Oct 2017

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