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DNA annealing

Optimization of the Mit1C real-time PCR assay using Bio-Rad CFX96 for the detection of Cyclospora cayetanensis in agricultural waters

Khai Truong, Miely Suarez, [ ... ], Gerardo U. Lopez

Dye-based qPCR assays for the separate quantification of total bacterial, fungal, arthropod, and plant DNA

Teresa M. Tiedge, Kim R. Love, Kelly A. Meiklejohn

A new molecular tool for detection of the highly invasive gecko, Hemidactylus frenatus

Arati Agarwal, Melissa L. Thomas, [ ... ], Mark J. Blacket

Contemporary eDNA methods complement conventional microscopy in zooplankton diet studies: Case study with American lobster postlarvae

Alexander Ascher, Peter D. Countway, [ ... ], Richard A. Wahle

Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome

Emily L. Bean, Janet L. Smith, Alan D. Grossman

DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

Seoee Lee, Hyunyoung Kim, [ ... ], Hyunju Ro

High-throughput Kinetics using capillary Electrophoresis and Robotics (HiKER) platform used to study T7, T3, and Sp6 RNA polymerase misincorporation

Zachariah I. Carter, William B. O’Brien, Sean Lund, Andrew F. Gardner

Developmental validation of the AGCU YNFS Y Kit: A new 6-dye multiplex system with 44 Y-STRs and 5 Y-InDels for forensic application

Chaoran Sun, Xindi Wang, [ ... ], Feng Song

Design and characterization of hyperactive mutants of the Agrobacterium tumefaciens telomere resolvase, TelA

Shu Hui Huang, Kayla Abrametz, Siobhan L. McGrath, Kerri Kobryn

Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1

Chunlin Peng, Benyi Fan, [ ... ], Liyun Huang

Effect of target gene sequence evenness and dominance on real-time PCR quantification of artificial sulfate-reducing microbial communities

Zhe Du, Sebastian F. Behrens

The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation

Mahrokh Balouchi, Shu Hui Huang, Siobhan L. McGrath, Kerri Kobryn

Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Ekaterina A. Maltseva, Inna A. Vasil’eva, [ ... ], Olga I. Lavrik

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