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Cell-free protein synthesis

Diaphorin, a polyketide produced by a bacterial endosymbiont of the Asian citrus psyllid, adversely affects the in vitro gene expression with ribosomes from Escherichia coli and Bacillus subtilis

Rena Takasu, Yuka Yasuda, Takashi Izu, Atsushi Nakabachi

published 14 Nov 2023

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Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses

Shogo Miyoshi, Soh Tokunaga, Tatsuhiko Ozawa, Hiroyuki Takeda, Mitsuo Aono, Takanori Miyoshi, Hiroyuki Kishi, Atsushi Muraguchi, Shin-ichi Shimizu, Akira Nozawa, Tatsuya Sawasaki

published 15 Apr 2020

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Cell-free synthesis of human toll-like receptor 9 (TLR9): Optimization of synthesis conditions and functional analysis

Srujan Kumar Dondapati, Georg Pietruschka, Lena Thoring, Doreen A. Wüstenhagen, Stefan Kubick

published 25 Apr 2019

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A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection

Takuya Kanno, Kaori Yasutake, Kazunori Tanaka, Shinji Hadano, Joh-E Ikeda

published 09 Oct 2017

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CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag

Hiroyuki Takeda, Wei Zhou, Kohki Kido, Ryoji Suno, Takahiro Iwasaki, Takuya Kobayashi, Tatsuya Sawasaki

published 25 May 2017

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Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems

Jurek Failmezger, Robert Nitschel, Andrés Sánchez-Kopper, Michael Kraml, Martin Siemann-Herzberg

published 19 Dec 2016

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Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives

Lena Thoring, Doreen A. Wüstenhagen, Maria Borowiak, Marlitt Stech, Andrei Sonnabend, Stefan Kubick

published 29 Sep 2016

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AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis

Tomoya Yano, Hiroyuki Takeda, Atsushi Uematsu, Satoshi Yamanaka, Shunsuke Nomura, Keiichirou Nemoto, Takahiro Iwasaki, Hirotaka Takahashi, Tatsuya Sawasaki

published 06 Jun 2016

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Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins

Hirotaka Takahashi, Atsushi Uematsu, Satoshi Yamanaka, Mei Imamura, Tatsuro Nakajima, Kousuke Doi, Saki Yasuoka, Chikako Takahashi, Hiroyuki Takeda, Tatsuya Sawasaki

published 01 Jun 2016

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Cell-Free Expression and In Situ Immobilization of Parasite Proteins from Clonorchis sinensis for Rapid Identification of Antigenic Candidates

Christy Catherine, Seung-Won Lee, Jung Won Ju, Ho-Cheol Kim, Hyun-Il Shin, Yu Jung Kim, Dong-Myung Kim

published 24 Nov 2015

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Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses

Emi Takamitsu, Motoaki Otsuka, Tatsuki Haebara, Manami Yano, Kanako Matsuzaki, Hirotsugu Kobuchi, Koko Moriya, Toshihiko Utsumi

published 26 Aug 2015

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Ultra-High-Throughput Screening of an In Vitro-Synthesized Horseradish Peroxidase Displayed on Microbeads Using Cell Sorter

Bo Zhu, Takuro Mizoguchi, Takaaki Kojima, Hideo Nakano

published 20 May 2015

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A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins

Toru Ezure, Kei Nanatani, Yoko Sato, Satomi Suzuki, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki Uozumi

published 08 Dec 2014

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