In general, point-of-care (POC) tests for Chlamydia trachomatis (Ct) show disappointing test performance, especially disappointing sensitivity results. However, one study sponsored by the manufacturer (Diagnostics for the Real World) reported over 80% sensitivity with their Chlamydia Rapid Test (CRT). We evaluated the performance of this CRT in a non–manufacturer-sponsored trial.
Between July 2009 and February 2010, we included samples from 912 women in both high- and low-risk clinics for sexually transmitted infections (STIs) in Paramaribo, Suriname. Sensitivity, specificity, positive- and negative predictive values (PPV and NPV) for CRT compared to NAAT (Aptima, Gen-Probe) were determined. Quantitative Ct load and human cell load were determined in all CRT and/or NAAT positive samples.
CRT compared to NAAT showed a sensitivity and specificity of 41.2% (95% CI, 31.9%–50.9%) and 96.4% (95% CI, 95.0%–97.5%), respectively. PPV and NPV were 59.2% (95% CI, 47.5%–70.1%) and 92.9% (95% CI, 91.0%–94.5%), respectively. Quantitative Ct bacterial load was 73 times higher in NAAT-positive/CRT-positive samples compared to NAAT-positive/CRT-negative samples (p<0.001). Human cell load did not differ between true-positive and false-negative CRT results (p = 0.835). Sensitivity of CRT in samples with low Ct load was 12.5% (95% CI, 5.2%–24.2%) and in samples with high Ct load 73.5% (95% CI, 59.9%–84.4%).
Citation: van der Helm JJ, Sabajo LOA, Grunberg AW, Morré SA, Speksnijder AGCL, de Vries HJC (2012) Point-of-Care Test for Detection of Urogenital Chlamydia in Women Shows Low Sensitivity. A Performance Evaluation Study in Two Clinics in Suriname. PLoS ONE 7(2): e32122. https://doi.org/10.1371/journal.pone.0032122
Editor: Deborah Dean, University of California San Francisco, University of California, Berkeley, and the Children's Hospital Oakland Research Institute, United States of America
Received: November 3, 2011; Accepted: January 19, 2012; Published: February 29, 2012
Copyright: © 2012 van der Helm et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Research and Development fund of the Municipal Health Service of Amsterdam [project no 2369 and 2371] and AGIS healthcare insurance [RVVZ no 1417000]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Urogenital chlamydia is the most prevalent, curable bacterial sexually transmitted infection (STI) worldwide , with a significant public health burden, especially in young women . The causative bacterium, Chlamydia trachomatis (Ct) causes a high rate of asymptomatic infections  and is associated with adverse outcomes like infertility, ectopic pregnancy and pelvic inflammatory disease (PID) . To reduce transmission and late complications, active case finding and early treatment are critical strategies. The standard diagnostics are Nucleic Acid Amplification Tests (NAAT), but they are expensive and require sophisticated laboratory conditions . This makes NAAT unsuitable for the detection of Ct for most low-resource settings . Therefore the World Health organization (WHO) has launched a priority program that is designated to develop affordable and reliable point-of-care (POC) tests for STIs that are predominant in low resource countries [http://www.who.int/std_diagnostics]. In this program, WHO has formulated the ASSURED criteria that POC tests have to meet: Affordable, Sensitive, Specific, User-friendly, Robust and rapid, Equipment-free, Deliverable to those who need them . The POC test result should be readily available, while the patient waits, to ensure prompt treatment. This is especially important where patient return for treatment is low. It is estimated that a POC test of moderate sensitivity (63%) combined with immediate treatment on-site may lead to the treatment of more infected individuals than an ultra-sensitive and specific NAAT alone when patient return is low . Moreover, counselling messages are most efficient when a diagnosis can be communicated during the same consultation . These advantages are relevant for industrialized countries as well, even if POC tests have a lower sensitivity than standard NAAT.
To date, POC tests for urogenital chlamydia show disappointing test characteristics, especially low sensitivity. In a recent evaluation, three POC tests for urogenital chlamydia, currently on the market, showed poor sensitivity between 12% and 17% in a non–manufacturer-sponsored clinical study . In contrast, one POC test for urogenital chlamydia (Diagnostics for the Real World, Cambridge, UK) especially developed for low-resource countries has an asserted sensitivity of over 80% . A manufacturer-sponsored diagnostic field study in the Philippines revealed sensitivities of 71% and 87% among women at high risk and low risk for STI, respectively . Suriname, South America, is a low-resource country and affordable and reliable diagnostics to detect Ct are urgently needed. Therefore, we aimed to evaluate the performance of this promising POC test in two outpatient clinics in Suriname, with the objective to use this test for intervention of the chlamydia epidemic.
Study sites and population
The study was approved by the ethical committee of the Ministry of Health of the Republic of Suriname (VG010-2007) and the ethical committee of the Academic Medical Centre, University of Amsterdam, the Netherlands (MEC07/127). Patients were recruited at two sites in Paramaribo, Suriname:
- The Dermatological Service, an integrated outpatient clinic that offers free-of-charge examination and treatment of STIs and infectious skin diseases like leprosy and leishmaniasis. All consecutive women who visited for an STI check-up were asked to participate in the study and were considered to be at high-risk for STI.
- The Lobi Foundation is a center for birth control and sexual health. As women who visit this clinic do not attend primarily to be checked for STI, these participants were considered to be at low risk for STI.
Recruitment took place between July 2009 and February 2010. Exclusion criteria were: use of antibiotics in the past 7 days, age younger than 18 years and previous participation. After written informed consent, patients were given a unique code to participate anonymously. Participants were interviewed about demographic characteristics, including self-reported ethnicity as Suriname is a multiethnic society, with many ethnic groups such as Creoles and Maroons (both descendants from the African diaspora due to slave trade), Hindustani, Javanese, and Chinese (all descendants from labor immigrants), Caucasians (descendants from Dutch farmers), indigenous Amerindian people and Mixed race persons. Moreover, participants were asked about willingness to wait for POC test results, although in our study participants did not receive the results from POC, and if they used any products for vaginal hygiene like douches, herbs, or other home products, and if so, in what frequency. Data were entered into an MS Access database.
Specimen collection and testing procedures
Nurse-collected vaginal swabs were obtained blindly for the Chlamydia Rapid Test (CRT) (Diagnostics for the Real World (Europe), Cambridge, UK) and NAAT (Aptima, Gen-Probe, San Diego, USA) testing using a cross-over model. This means that in the first half of the included women the swab for the CRT was taken first and the second of the included women NAAT was taken first. Nurses were trained to collect the swabs before routine speculum examination was performed. A minimum period of 10 times for CRT and 10 seconds for NAAT of contact between the tip of the swab and the vaginal wall in a rotating motion was ensured. CRT was immediately performed according to the manufacturer's instructions on-site in the laboratory. All technicians that performed the CRT were trained with proficiency panels as provided and instructed by the manufacturer. Technicians did not receive information about the participant. The test results were interpreted and recorded by two laboratory technicians separately. CRT results were defined as indeterminate when the laboratory technicians reported discordant results or when CRT failed (i.e. control line did not appear). The samples for NAAT testing were collected according to the manufacturer's instructions, and shipped to the Public Health Laboratory in Amsterdam where they were tested within 50 days after collection. NAAT test results were communicated with the two recruitment sites in Suriname and participants with a positive-Ct NAAT were treated with doxycycline 100 mg bid for 7 days at Lobi Foundation and 10 days bid at the Dermatological Service or, in case of (possible) pregnancy, with a single 1000 mg oral dose of azithromycin.
Chlamydia Rapid Test
The CRT was performed as described previously . Version 6.1 of the Chlamydia Rapid Test (Professional use) (P/N 1200-20) instructions for use (C03-0008) was used. Shortly, each swab was subjected to extraction by sequential addition of 400 µl of reagent 1, 300 µl of reagent 2, and 100 µl of reagent 3 to the swab in a tapered sample preparation tube, with gentle mixing between additions. The sample preparation reagents were administered with unit dose pipettes. The extraction tube was then capped and used as a dropper to deliver 5 drops (approximately 100 µl) of the extracted sample to a tube containing the lyophilized amplification and detection reagents. The resulting mixture was agitated gently until a clear pink solution was obtained, after which the test strip, coated with a monoclonal antibody to chlamydial lipopolysaccharide (LPS) and including a procedural control, was added to the solution and allowed to stand for 25 minutes before the result was read. Each swab was subjected to one extraction. The test strip was used in the interpretation of the result; a clearly visible test line indicated a positive result, provided that the control line was also visible on the test strip.
For NAAT testing, the monospecific Aptima chlamydia assay for the detection of Chlamydia trachomatis rRNA (Gen-Probe Inc., San Diego, USA) was used with the accompanying vaginal swab specimen collection kit. The protocols described in the package inserts were followed. Technicians performing NAAT were blinded to the results of the POC-Ct and did not receive clinical information. This NAAT is an FDA-approved commercial test and was used to estimate the Ct prevalence at both study sites.
Quantitation of Ct load and HLA
Quantitative Ct load was determined for samples with a discrepant test result between CRT and NAAT, and for samples that tested positive for CRT as well as for NAAT using a real-time PCR targeting the cryptic plasmid . Ct load was expressed as inclusion forming units (IFU) based on defined serial dilutions of Ct cultured in human cells with over >90% infected HeLa cells of 100 IFU to 0.001 IFU taking into account also DNA from non-viable Ct particles. The human cell load was assessed by determination of human HLA copies in combination with a defined serial dilution of quantified human DNA using the following primer probe combination: HLA-F 5′-TTG-TAC-CAG-TTT-TAC-GGT-CCC-3′ HLA-R 5′- TGG-TAG-CAG-CGG- TAG-AGT-TG,-3 and HLA-Probe 5′-FAM- TTC TAC GTG GAC CTG GAG AGG AAG GAG -BHQ1-3′. By using a chlamydial and a human target, the average chlamydial/human cell ratio, and IFU/swab were calculated .
To evaluate the performance of CRT compared to NAAT sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated using standard methods. Specimens with indeterminate results by CRT were excluded. An independent t-test was used to compare log-transformed Ct loads between true-positive and false-negative CRT results. Analyses were performed with SPSS package version 19.0 (SPSS Inc., Chicago, IL).
The study has been reported according to the STARD checklist for the reporting of studies of diagnostic accuracy.
Study population and specimens
In total, 1019 women were asked to participate in the study, of whom 917 were included and 102 did not meet the inclusion criteria or declined to participate (Figure 1). Five women were excluded from the CRT performance evaluation due to either discrepancy in CRT result between two lab technicians (n = 3) or failure of CRT (n = 2).
NAAT; Aptima chlamydia single test, Genprobe (control test) CRT; Chlamydia Rapid Test, Diagnostics for the Real World (evaluated test).
General characteristics of the 912 women included in the CRT performance evaluation are shown in Table 1. Their median age was 30 years (IQR 25–36), 336 (36.9%) were of Creole/Maroon ethnicity and 229 (25.1%) were of Hindustani ethnicity. Twenty-one (2.3%) women reported having had sex for money or goods. Almost all women 900 (98.7%) would wait for the CRT test result if the test were a standard offering in their clinic. Of these women, 660 (73.3%) would be willing to wait for a maximum of half an hour to receive the results, the other 240 (26.7%) would be willing to wait for at least an hour.
Ct prevalence and CRT performance results
Ct prevalence was 20.8% in the high-risk population (visiting the Dermatological Service) and 9.2% in the low-risk population (visiting Lobi Foundation). Combining the results of the two sites, the sensitivity and specificity of the CRT in identifying Ct compared to NAAT were 41.2% (95% CI, 31.9%–50.9%) and 96.4% (95% CI, 95.0%–97.5%), respectively. PPV of the CRT was 59.2% (95% CI, 47.5%–70.1%) and NPV was 92.9% (95% CI, 91.0%–94.5%). Sensitivity and specificity of CRT compared to NAAT were comparable for the high-risk population (39.4% and 94.4%) and the low-risk population (42.0% and 96.8%) (Table 2).
Quantitative load measurements
Quantitative Ct bacterial load and human HLA were assessed for the samples that showed discrepant results for CRT and NAAT (n = 89) and for samples that were CRT and NAAT positive (n = 42). Ct bacterial load could be detected in 99/131 samples and human HLA in 126/131 samples. Of the 42 samples that tested positive for CRT and NAAT, quantitative Ct bacterial load was detected in all 42 samples and human HLA in 39 samples. Of the 60 samples that tested CRT negative and NAAT positive, quantitative Ct bacterial load was detected in 55 samples and human HLA in all 60 samples. Of the 29 samples that tested CRT positive and NAAT negative, quantitative Ct bacterial load was detected in 2 samples and human HLA in 27 samples (Table 3).
Quantitative Ct bacterial load was 73 times higher in NAAT-positive/CRT-positive samples (geometric mean 120 IFU) compared to NAAT-positive/CRT-negative samples (geometric mean 1.64 IFU, p<0.001). Human DNA concentration did not differ between the true-positive and false-negative CRT results (p = 0.835). The average chlamydial/human cell load ratio (Ct concentration) was 60 times higher in NAAT-positive samples where CRT detected Ct infection (geometric mean 0.32 IFU/human cell) compared to loads that CRT did not detect (geometric mean 0.0053 IFU/human cell, p<0.001). Quantitative HLA load was comparable for NAAT-positive/CRT-positive samples (geometric mean 344 cells) compared to NAAT-negative/CRT-positive samples (geometric mean 451 cells, p = 0.424).
Quantitative Ct loads were comparable for women reporting symptoms like vaginal discharge, irregular menstruation, pain during intercourse, lower abdominal pain or dysuria and women without the specific symptom (data not shown). Women visiting the high-risk STI clinic had comparable quantitative Ct loads with those visiting the low-risk clinic (p = 0.525). Sensitivity of the CRT was comparable for those who practiced any vaginal hygienic measures, 37.5% (95% CI, 23.6%–53.1%), compared to those who did not practice vaginal cleansing, 43.3% (95% CI, 31.3%–56.0%). When comparing women who practice vaginal cleansing frequently, at least once a week, with those who cleanse less than once weekly, sensitivity of CRT yields comparable results, 39.1% (95% CI, 21.1%–59.8%) and 27.3% (95% CI, 7.5%–57.8%), respectively.
Based on the overall median Ct load, NAAT-positive samples were divided in two groups with either a low- (range 0.006–12.5 IFU) or high-grade quantitative bacterial Ct load (range 13.5–6470 IFU). In the low-grade bacterial load group, the CRT sensitivity was 12.5% (95% CI, 5.2%–24.2%), whereas in the high-grade Ct load group the sensitivity was 73.5% (95% CI, 59.9%–84.4%).
We found a disappointingly low clinical sensitivity of 42.0% and 39.4% of the CRT in low-risk and high-risk Surinamese women, respectively, compared to the sensitivity of 86.8% in low-risk women and 71% in high-risk women in the Philippines, reported earlier in a study supported by the manufacturer . The discrepancy might partly be explained by the use of a different reference test. Where we used Gen-Probe's Aptima platform as a reference test, in the Philippines study the Roche Amplicor (Roche Molecular Systems, Branchburg, NJ) was used. Although current generation NAATs have comparable sensitivities, NAAT could be more sensitive than Roche Amplicor . A somewhat lower sensitivity of CRT in our study could be expected with a more sensitive control test, but this does not explain the large difference in sensitivity found in the Philippine study and our results.
Another explanation for the lower sensitivity we found could be attributed to a different wash-out period for antibiotic use between the two studies. We excluded women who used antibiotics in the last 7 days, while in the Philippines study women who used antibiotics in the previous month were excluded. Time to clearance of LPS antigen, which is targeted by the CRT, might be shorter after antibiotic use than time to clearance of Ct rRNA, which is targeted by NAAT . This could have caused the occurrence of false-positive NAAT samples, and consequently more false-negative CRT samples could be expected. Low sensitivity of the CRT due to inadequate collection resulting in a low sample yield could be ruled out since the human cell load in samples with true-positive and false-negative CRT results was comparable. The CRT had a 96.4% specificity. False-positive CRT results could have been caused by cross reactivity with C. ptsittaci or C. pneumoniae as described in the manufacturers manual. Yet infections with these organisms in the urogenital tract in humans are uncommon , . As a false positive chlamydia diagnosis can have serious adverse social consequences a specificity of 96,4% is undesirable, especially in low prevalent settings. The CRT in our study had a few modifications compared to the study in the Philippines. We used unit dose pipettes instead of unit dose vials. Also, the nitrocellulose membrane was changed by the manufacturer and according to the manual, only one dipstick had to be used to interpret the results. However, when a test is renewed one might expect at least comparable diagnostic characteristics compared to the previous test.
In the CRT evaluation study performed in the Philippines, the Ct prevalence was 6.3% in the low-risk group (women visiting an obstetrics-gynaecology clinic) and 17.9 to 32% in the high-risk group (female sex workers), which compares well with the prevalences found in our study, 9.2% and 20.8% respectively. The sensitivity figures found in our study were comparable for low-risk and high-risk women, 42.0% and 39.4% respectively. Quite surprisingly, in the Philippines study a much lower sensitivity was found in the high-risk group compared to the low-risk group. The authors explain this finding as a result of the use of vaginal creams and other feminine hygiene products, which can interfere with the CRT. In our study, the sensitivity of CRT was comparable for women who practiced any vaginal cleansing and those who did not.
Although we consider the population recruited at Lobi Foundation a low risk group, with a prevalence of 9.2% this population would be considered high risk in many settings. Yet, with a prevalence of 20.8% as found at the Dermatological Service, the difference in prevalence between the two study sites is substantial.
The sensitivity of CRT is higher in samples with a high bacterial load. The clinical relevance of organism load is still debated, but it is suggested that infections with high organism loads are more likely to lead to cervicitis or PID and are associated with multiple patient-reported symptoms . However, the association with patient-reported symptoms was only found with first-void urine and endocervical samples and not with self-collected vaginal samples. In our study, where nurse-collected vaginal swabs were used, quantitative Ct loads were not significantly different for asymptomatic women and women reporting one or multiple symptoms such as vaginal discharge or dysuria.
The NAAT platform is a latest generation highly sensitive commercial diagnostic test for Ct . However no test is 100% accurate and a positive bacterial Ct load signal was detected in two samples that were NAAT negative and CRT positive. One sample had a Ct load of 62.9 IFU which might be explained by inhibition of high target load . The other sample had a very low load of 0.00261 IFU. Since the frequency of these discrepancies was extremely low, we do not consider that this finding significantly affects our test evaluation.
A recent field study of the same CRT test but to detect ocular chlamydia infection (trachoma) found similar disappointingly low sensitivity (33.3%–67.9%) and specificity (92.4%–99.0%) . Most commercially available and clinically evaluated POC tests for urogenital chlamydia show poor sensitivity results . Compared with the results found in our evaluation, the CRT of Diagnostics for the Real World outperforms some of the other commercially available products . Still, with a sensitivity of only 41.7%, this test performs under the minimally required sensitivity of 63% required for a POC test to treat more infected individuals than the standard NAAT in a setting with low patient return (<65%), . On the other hand, in situations where transmission during treatment delay and low return for treatment are considerable, even a POC test with a sensitivity below 63% could be beneficial in the prevention of ongoing STI transmission . A recent economic evaluation analysis using the same CRT as we evaluated in this study, showed that in the UK using NAAT is more cost-effective. . In that evaluation, a sensitivity between 73% and 85% for the CRT was assumed.
POC tests available for systemic infections like HIV and syphilis are highly sensitive since they are based on the detection of serum antibodies , . Infections caused by organisms like Ct (but also N. gonorrhoeae) are confined to mucosal tissue and normally invoke little to no production of antibodies. Therefore, the development of POC tests to diagnose mucosal Ct infections based on the detection of serum antibodies is, at least for now, not an option. Improved POC tests for Ct need to detect bacterial antigens or nucleic acids, even in cases with a low bacterial load. Promising steps have been made in the field of POC HIV-load NAAT using nanotechnology . Along the same lines, a POC test for urogenital chlamydia with sufficient sensitivity could be developed. Until reliable and affordable diagnostics are available, algorithms for syndromic management can be used for low-resource settings, although the success of algorithms for vaginal discharge varies between populations .
In conclusion, the evaluated CRT of Diagnostics for the Real World has no added value in the management of Ct infections due to its low test performance. There is an urgent need for POC diagnostics for the detection of urogenital chlamydia meeting the ASSURED criteria, including adequate sensitivity.
The authors would like to express their gratitude to all nurses and laboratory technicians of the Dermatological Service and the Lobi Foundation for data collection, and Susan T. Landry for editing the final manuscript.
Conceived and designed the experiments: JvdH LS SM AS HdV. Performed the experiments: JvdH AG SM AS. Analyzed the data: JvdH SM HdV. Contributed reagents/materials/analysis tools: LS AG SM AS HdV. Wrote the paper: JvdH LS AG SM HdV.
- 1. World Health OrganizationGlobal prevalence and incidence of selected curable sexually transmitted infections: Overview and estimates. Geneva, Switzerland: World Health Organization, 2001. Available: http://www.who.int/hiv/pub/sti/pub7/en/ Accessed 2012 Jan 30.
- 2. Datta SD, Sternberg M, Johnson RE, Berman S, Papp JR, et al. (2007) Gonorrhea and chlamydia in the United States among persons 14 to 39 years of age, 1999 to 2002. Ann Intern Med 147: 89–96.
- 3. Farley TA, Cohen DA, Elkins W (2003) Asymptomatic sexually transmitted diseases: the case for screening. Prev Med 36: 502–509.
- 4. Land JA, van Bergen JE, Morre SA, Postma MJ (2010) Epidemiology of Chlamydia trachomatis infection in women and the cost-effectiveness of screening. Hum Reprod Update 16: 189–204.
- 5. Peeling RW, Mabey D, Herring A, Hook EW (2006) Why do we need quality-assured diagnostic tests for sexually transmitted infections? Nat Rev Microbiol 4: S7–19.
- 6. Petti CA, Polage CR, Quinn TC, Ronald AR, Sande MA (2006) Laboratory medicine in Africa: a barrier to effective health care. Clin Infect Dis 42: 377–382.
- 7. Peeling RW, Holmes KK, Mabey D, Ronald A (2006) Rapid tests for sexually transmitted infections (STIs): the way forward. Sex Transm Infect 82: Suppl 5v1–v6.
- 8. Gift TL, Pate MS, Hook EW, Kassler WJ (1999) The rapid test paradox: when fewer cases detected lead to more cases treated: a decision analysis of tests for Chlamydia trachomatis. Sex Transm Dis 26: 232–240.
- 9. Sanders GD, Anaya HD, Asch S, Hoang T, Golden JF, et al. (2010) Cost-effectiveness of strategies to improve HIV testing and receipt of results: economic analysis of a randomized controlled trial. J Gen Intern Med 25: 556–563.
- 10. van Dommelen L, van Tiel FH, Ouburg S, Brouwers EE, Terporten PH, et al. (2010) Alarmingly poor performance in Chlamydia trachomatis point-of-care testing. Sex Transm Infect 86: 355–359.
- 11. Mahilum-Tapay L, Laitila V, Wawrzyniak JJ, Lee HH, Alexander S, et al. (2007) New point of care Chlamydia Rapid Test–bridging the gap between diagnosis and treatment: performance evaluation study. BMJ 335: 1190–1194.
- 12. Saison F, Mahilum-Tapay L, Michel CE, Buttress ND, Nadala EC, et al. (2007) Prevalence of Chlamydia trachomatis infection among low- and high-risk Filipino women and performance of Chlamydia rapid tests in resource-limited settings. J Clin Microbiol 45: 4011–4017.
- 13. Michel CE, Solomon AW, Magbanua JP, Massae PA, Huang L, et al. (2006) Field evaluation of a rapid point-of-care assay for targeting antibiotic treatment for trachoma control: a comparative study. Lancet 367: 1585–1590.
- 14. Catsburg A, Savelkoul PHM, Vliet A, Algra J, Vandenbroucke-Grauls CMJE, et al. (2006) Development and evaluation of an internally controlled Real-Time quantitative PCR assay for the detection of Chlamydia trachomatis. In: Chernesky M, Caldwell H, Christiansen G, et al., editors. pp. 521–524. Eleventh International Symposium on Human Chlamydial Infections. June 18–23, 2006, Niagara-on-the-Lake, Ontario, Canada.
- 15. Lowe P, O'Loughlin P, Evans K, White M, Bartley PB, et al. (2006) Comparison of the Gen-Probe APTIMA Combo 2 assay to the AMPLICOR CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples from Australian men and women. J Clin Microbiol 44: 2619–2621.
- 16. Renault CA, Israelski DM, Levy V, Fujikawa BK, Kellogg TA, et al. (2011) Time to clearance of Chlamydia trachomatis ribosomal RNA in women treated for chlamydial infection. Sex Health 8: 69–73.
- 17. Pospischil A, Thoma R, Hilbe M, Grest P, Gebbers JO (2002) Abortion in woman caused by caprine Chlamydophila abortus (Chlamydia psittaci serovar 1). Swiss Med Wkly 132: 64–66.
- 18. Whyte A, Garnett P, Thompson C, McMullen P (1998) Chlamydia pneumoniae in the female genital tract. J Infect 36: 245.
- 19. Michel CE, Sonnex C, Carne CA, White JA, Magbanua JP, et al. (2007) Chlamydia trachomatis load at matched anatomic sites: implications for screening strategies. J Clin Microbiol 45: 1395–1402.
- 20. Chernesky M, Jang D, Luinstra K, Chong S, Smieja M, et al. (2006) High analytical sensitivity and low rates of inhibition may contribute to detection of Chlamydia trachomatis in significantly more women by the APTIMA Combo 2 assay. J Clin Microbiol 44: 400–405.
- 21. Birch L, Dawson CE, Cornett JH, Keer JT (2001) A comparison of nucleic acid amplification techniques for the assessment of bacterial viability. Lett Appl Microbiol 33: 296–301.
- 22. Harding-Esch EM, Holland MJ, Schemann JF, Molina S, Sarr I, et al. (2011) Diagnostic accuracy of a prototype point-of-care test for ocular Chlamydia trachomatis under field conditions in The Gambia and Senegal. PLoS Negl Trop Dis 5: e1234.
- 23. Vickerman P, Watts C, Alary M, Mabey D, Peeling RW (2003) Sensitivity requirements for the point of care diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae in women. Sex Transm Infect 79: 363–367.
- 24. Hislop J, Quayyum Z, Flett G, Boachie C, Fraser C, et al. (2010) Systematic review of the clinical effectiveness and cost-effectiveness of rapid point-of-care tests for the detection of genital chlamydia infection in women and men. Health Technol Assess 14: 1–iv.
- 25. Delaney KP, Branson BM, Uniyal A, Phillips S, Candal D, et al. (2011) Evaluation of the performance characteristics of 6 rapid HIV antibody tests. Clin Infect Dis 52: 257–263.
- 26. Chin CD, Laksanasopin T, Cheung YK, Steinmiller D, Linder V, et al. (2011) Microfluidics-based diagnostics of infectious diseases in the developing world. Nat Med 17: 1015–1019.
- 27. Niemz A, Ferguson TM, Boyle DS (2011) Point-of-care nucleic acid testing for infectious diseases. Trends Biotechnol 29: 240–250.
- 28. Pettifor A, Walsh J, Wilkins V, Raghunathan P (2000) How effective is syndromic management of STDs?: A review of current studies. Sex Transm Dis 27: 371–385.