Peer Review History
Original SubmissionSeptember 28, 2020 |
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PONE-D-20-30493 Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources PLOS ONE Dear Dr. Kim, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Dec 20 2020 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. Please include the following items when submitting your revised manuscript:
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Kind regards, Himanshu Sharma Academic Editor PLOS ONE Additional Editor Comments: Authors have done good work, for the genetic improvement in buckwheat, and the manuscript entitled "Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources" has been extensively reviewed by the reviewers, They suggested revision of the manuscript, So the authors extensively revised the manuscript according to the suggestions of the reviewer.So there are chances of the improvement in the current manuscript. Journal Requirements: When submitting your revision, we need you to address these additional requirements. 1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and 2. 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PLOS ONE now requires that authors provide the original uncropped and unadjusted images underlying all blot or gel results reported in a submission’s figures or Supporting Information files. This policy and the journal’s other requirements for blot/gel reporting and figure preparation are described in detail at https://journals.plos.org/plosone/s/figures#loc-blot-and-gel-reporting-requirements and https://journals.plos.org/plosone/s/figures#loc-preparing-figures-from-image-files. When you submit your revised manuscript, please ensure that your figures adhere fully to these guidelines and provide the original underlying images for all blot or gel data reported in your submission. See the following link for instructions on providing the original image data: https://journals.plos.org/plosone/s/figures#loc-original-images-for-blots-and-gels. In your cover letter, please note whether your blot/gel image data are in Supporting Information or posted at a public data repository, provide the repository URL if relevant, and provide specific details as to which raw blot/gel images, if any, are not available. Email us at plosone@plos.org if you have any questions. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Partly ********** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: N/A Reviewer #3: Yes ********** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Yes ********** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: No Reviewer #2: No Reviewer #3: Yes ********** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: Comments on the manuscript PONE-D-20-30493 The manuscript PONE-D-20-30493, entitled “Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources”, developed 50 InDels to instruct a barcode system to increase the genetic diversity of tartary buckwheat. The results are interesting for identification and assessment of genetic diversity of tartary buckwheat resources. However, there are some problems in this manuscript. I suggest that this manuscript should make a major revision before being accepted for publication. 1. The language of this manuscript should be improved by a native English speaker, or a professional language editing service. There are a lot of grammar errors in this manuscript, such as Lines 94-97. 2. The 50 InDel-based barcode system has a bias for genetic identification of tartary buckwheat resources. The amount of 50 InDel-based genotypes of 73 accessions is not enough. Reviewer #2: Dear Authors, You have generated substantial information on developing INDEL barcode system in tartary buckwheat which can be improve the reliability of identifying genetic diversity among buckwheat germplasm. I found this study quite helpful in targeting future buckwheat breeding programs but have some minor concerns before formally accepting it from my end: Abstract has been casually written in terms of language used, especially lines 41-45. Please modify accordingly. What age plant material was used for measuring the rutin and quercetin contents? Mature….how many years? What do you mean by ‘etc.’ in Fig. 2? I am sorry but you can’t use such terms especially describing a figure. Correct it in figure legend as well as in the figure itself. You can add country names as and what available. In Fig. 3, replace the word ‘InDel’ by ‘InDels’ as you are dealing with a large no. References need thorough rechecking as per journal standards. Maintain uniformity while citing edited books, chapters and/or proceedings. Lastly, rectify the grammatical mistakes and sentence framing. Authors seemed to be in a hurry while compiling this analysis. It will be better if help of some native English speaker could be taken. Regards Reviewer #3: In this manuscript, the authors used combination of Pacbio long reads and Illumina short reads for draft genome assembly of tartary buckwheat cv. ‘Daegwan 3-7a and through whole-genome resequencing of 26 accessions had identified 171,926 homogeneous and 53,755 heterogeneous InDels. The technical approach chosen for this study is sound and the processing steps presented are commonly used for this kind of study. However, there are few major concerns in the current manuscript, which authors should address A. The authors write that they have represented a high quality draft genome assembly and annotation for tartary buckwheat. They do not provide the needed analysis results to backup this assertion, as they have not compared their assembly with previously available assemblies. B. There is a need to include a large number of accessions representing any countries and indels for genetic identification of tartary buckwheat resources. There are also some minor points need to be addressed 1. The material and methods section of the manuscript is not precise enough and does not contain all the processing steps presented in the result section. Such as the conditions for structure analysis should be detailed, with parameter settings. 2. The manuscript needs to be rechecked for grammatical errors and spelling mistakes. 3. Resolution of the all the figures should be checked to comply quality parameters of the journal. 4. In figure 5 A author must include a DNA base pair ladder. 5. In Figure 8 B there is a need to represent the differentiate accessions in their respective subgroups by encircling them. ********** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: No Reviewer #2: No Reviewer #3: Yes: Pradeep Singh [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. |
Revision 1 |
PONE-D-20-30493R1 Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources PLOS ONE Dear Dr. Yul-Ho Kim Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Apr 09 2021 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. Please include the following items when submitting your revised manuscript:
If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter. If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols We look forward to receiving your revised manuscript. Kind regards, Himanshu Sharma Academic Editor PLOS ONE Journal Requirements: Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice. Additional Editor Comments (if provided): Based on the recommendations of the reviewer's authors had significantly revised the manuscript, and by analyzing the the revised manuscript the suggested that manuscript can be accepted for publication. But my question is that authors have not deposit the sequencing data in Public repositories like NCBI or any other. Firstly authors submit data into NCBI and put SRA number or any ID, Because I don't get the details for the same in the manuscript and I am not satisfied with this, I only accept the manuscript if authors provide these details. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation. Reviewer #1: All comments have been addressed Reviewer #2: All comments have been addressed Reviewer #3: All comments have been addressed ********** 2. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Yes ********** 3. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Yes ********** 4. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Yes ********** 5. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: Yes Reviewer #3: Yes ********** 6. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: The authors have revised their manuscript according to the reviewers' comments. So I suggest that the manuscript should be accepted now. Reviewer #2: All the concerned queries have been addressed satisfactorily. While language was a constraint initially, it has also been improved. Reviewer #3: (No Response) ********** 7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: No Reviewer #2: Yes: Dr. Nikhil Malhotra, ICAR-National Bureau of Plant Genetic Resources Regional Station, Shimla, India Reviewer #3: Yes: Pradeep Singh [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.
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Revision 2 |
Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources PONE-D-20-30493R2 Dear Dr. Kim, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Himanshu Sharma Academic Editor PLOS ONE Additional Editor Comments (optional): Authors have addressed all the queries raised by reviewer and Academic Editor and putting one note that in future when this genome sequence is annotated and completed they must submit the sequence in public repositories. Now the manuscript is looking good, but there are always possibilities of correcting the mistakes which authors will take care at the time of proofread. Reviewers' comments: |
Formally Accepted |
PONE-D-20-30493R2 Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources Dear Dr. Kim: I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. If we can help with anything else, please email us at plosone@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Himanshu Sharma Academic Editor PLOS ONE |
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