PONE-D-19-26779

Clinical evaluation of postoperative analgesia and serum biochemical changes of paracetamol compared to meloxicam and carprofen in bitches undergoing ovariohysterectomy

PLOS ONE

Dear José A Ibancovichi

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1. At this time, we request that you  please report additional details in your Methods section regarding animal care, as per our editorial guidelines:

(1) Please state the breeds of the dogs used in this study  

(2) Please provide details of animal welfare (e.g., shelter, food, water, environmental enrichment)

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2. Thank you for providing a copy of the criteria used in the University of Melbourne's Pain Scale (UPMS). At this time, we also ask that you please provide a copy of the Dynamic Interactive Visual Analog Scale (DIVAS) socring system if is not under a copyright more restrictive than CC-BY. Please include a copy, in both the original language and English, as Supporting Information to ensure that you have provided sufficient details that others could replicate the analyses.

3. Thank you for stating that consent was obtained from the owners of the female dogs used in this study. At this time, we also ask that you please provide additional details regarding owner consent. Please ensure that you have specified (1) whether consent was informed and (2) what type you obtained (for instance, written or verbal, and if verbal, how it was documented and witnessed).

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Reviewer #1: Yes

Reviewer #2: Partly

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Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Multimodal analgesia becoming very popular all for the purpose of decreasing pain and avoid the side effect of narcotics.

In conclusion there was no significant difference between the 3 drugs due limited number of the cases in the study and lack of serum concentration of the 3 different drugs.

Reviewer #2: This study is a pain study in female dogs who had hysterectomy and salpingopherectomy. The authors compared the analgesic effect of paracetamol, carboprofen or meloxicam for postoperative pain management and biochemical changes.

It think it is better to replace the biochemical changes with liver and kidney function tests and the hemodynamics. Biochemical changes imply that the blood levels of medications will be measured.

Sample size calculation needs to be included. It is hard to draw conclusions from a study of 10 patients in each group.

I'm not sure why did the study look at the liver and kidney functions while using the therapeutic doses of the medications and expect to have a difference . The only difference that was reported was slight increase of the ALT on day 4 .

The study also, looked at the hemodynamic changes of medications that are known to not cause any significant changes. The only reported a significant change was a decrease of MAP in the carpoprofen group. They found the MAP in the carboprofen group to be 71+/- 14 compared to 82+/- 17 and 84+/- 16 which is still in the acceptable range.

The introduction and the discussion should be shortened.

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Reviewer #1: Yes: John Seif MD, MBA

Reviewer #2: No

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Clinical evaluation of postoperative analgesia, cardiorespiratory parameters and changes in liver and renal function tests of paracetamol compared to meloxicam and carprofen in dogs undergoing ovariohysterectomy

PONE-D-19-26779R1

Dear Dr. José A Ibancovichi

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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With kind regards,

Ehab Farag, MD FRCA FASA

Academic Editor

PLOS ONE

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