Fig 1.
A rooted ML tree based on 16S rRNA gene nucleotide sequences of strain PT3T and related type strains.
Numbers shown on branches are bootstrap values (>50%) based on 1,000 replicated analysis from maximum-likelihood algorithm. Bar, 0.1 substitutions per nucleotide position. Sequences trimmed to 1,337 bp were compared (54–1,390 position in Al. ceti RA1T, KJ867528). Escherichia coli K-12 was used as an outgroup.
Fig 2.
AAI matrix using Aliamphritea and related Oceanospirillaceae.
Reference genomes were downloaded from NCBI database (S2 Table).
Fig 3.
A list of strains and their assembly accession is provided in S2 Table.
Table 1.
Phenotypic characteristics of PT3T and related species from the genus Amphritea.
Table 2.
Phenotypic characteristics of Aliamphritea and genus level comparison within the family Oceanospirillaceae.
Fig 4.
Anvi’o representation of the pangenome of the Aliamphritea and Amphritea species.
Layers represent each genome, and the bars represent the occurrence of gene clusters. The darker colored areas of the bars belong to one of the three bins: Core, Aliamphritea unique or Amphritea unique.
Fig 5.
Syntenic dotplot comparison of Aliamphritea and Amphritea type strains.
Dots closer to the diagonal line represents collinear arrangement between two homologous genes in two genomes. (A), (B) Intra-genus comparison of Aliamphritea species. (C), (D) Inter-genus comparison between Amphritea and Aliamphritea species. (E) Intra-genus comparison of Amphritea species.
Fig 6.
Predicted fatty acid synthetic pathway in Amphritea and Aliamphritea species.
Genes encoding each enzyme were present in all strains unless stated otherwise. *1: Only present in Al. hakodatensis, Al. ceti and Al. spongicola. *2: Only present in Al. ceti, A. atlantica, A. japonica, A. balenae and A. pacifica. ACP: acyl-carrier protein; AccABCD: acetyl-CoA carboxylase complex; FabD: malonyl-CoA: ACP transacylase; FabH/FabY: 3-ketoacyl-ACP synthase Ⅲ; FabB: 3-ketoacyl-ACP synthase Ⅰ; FabF: 3-ketoacyl-ACP synthase Ⅱ; FabG: 3-ketoacyl-ACP reductase; FabA: 3-hydroxyacyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase; enoyl-ACP reductase; FabZ: 3-hydroxyacyl-ACP dehydratase.
Table 3.
FAS associated genes composition of the eight strains.