Table 1.
Sampling information for the eDNA spatial transect (sampled 20.09.2018).
Fig 1.
Known distribution of Elodea canadensis in Norway and survey locations.
In A–B The red triangular symbols represent localities with known presence of Elodea canadensis downloaded from Artsdatabanken (https://artsdatabanken.no/), and the green diamond symbols indicate the sites for the visual survey. In C–D the blue circle symbols show water sample locations for eDNA analysis (with ID numbers #, see Table 1).
Table 2.
Primers and probe.
Fig 2.
Alignment of relevant Hydrocharitaceae sequences obtained by performing a BLAST search using the Elodea canadensis product sequence amplified from a locus on the intergenic spacer between trnL and trnF. Species specific oligonucleotides for E. canadensis and Egeria densa (Planch.) [32] are shown at the bottom.
Fig 3.
eDNA spatial transect over the Leira catchment.
Numeration corresponds to Fig 1 and Table 1. Technical qPCR replicates are shown for each sampling site.
Fig 4.
Elodea canadensis eDNA time series at Lake Steinsfjorden (one location).
No surface eDNA samples were collected on the 20 June and 18 July 2016. Filtered volume for all samples was 5000 mL apart for the 10th October 2016 bottom sample that used only 750 mL due to high turbidity. Technical qPCR replicates (parallels) are shown for each sampling date and depth.