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Detection of an invasive aquatic plant in natural water bodies using environmental DNA

  • Marc B. Anglès d’Auriac ,

    Roles Data curation, Formal analysis, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

    Affiliation Norwegian Institute for Water Research (NIVA), Oslo, Norway

  • David A. Strand,

    Roles Formal analysis, Investigation, Methodology, Project administration, Writing – review & editing

    Affiliations Norwegian Institute for Water Research (NIVA), Oslo, Norway, Norwegian Veterinary Institute, Oslo, Norway

  • Marit Mjelde,

    Roles Conceptualization, Investigation, Writing – review & editing

    Affiliation Norwegian Institute for Water Research (NIVA), Oslo, Norway

  • Benoit O. L. Demars,

    Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

    Affiliation Norwegian Institute for Water Research (NIVA), Oslo, Norway

  • Jens Thaulow

    Roles Conceptualization, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Norwegian Institute for Water Research (NIVA), Oslo, Norway

Detection of an invasive aquatic plant in natural water bodies using environmental DNA

  • Marc B. Anglès d’Auriac, 
  • David A. Strand, 
  • Marit Mjelde, 
  • Benoit O. L. Demars, 
  • Jens Thaulow


The ability to detect founding populations of invasive species or rare species with low number of individuals is important for aquatic ecosystem management. Traditional approaches use historical data, knowledge of the species’ ecology and time-consuming surveys. Within the past decade, environmental DNA (eDNA) has emerged as a powerful additional tracking tool. While much work has been done with animals, comparatively very little has been done with aquatic plants. Here we investigated the transportation and seasonal changes in eDNA concentrations for an invasive aquatic species, Elodea canadensis, in Norway. A specific probe assay was developed using chloroplast DNA to study the fate of the targeted eDNA through space and time. The spatial study used a known source of Elodea canadensis within Lake Nordbytjern 400 m away from the lake outlet flowing into the stream Tveia. The rate of disappearance of E. canadensis eDNA was an order of magnitude loss over about 230 m in the lake and 1550 m in the stream. The time series study was performed monthly from May to October in lake Steinsfjorden harbouring E. canadensis, showing that eDNA concentrations varied by up to three orders of magnitude, peaking during fall. In both studies, the presence of suspended clay or turbidity for some samples did not hamper eDNA analysis. This study shows how efficient eDNA tools may be for tracking aquatic plants in the environment and provides key spatial and temporal information on the fate of eDNA.


The detection of invasive species is often challenging during the initial settlement phase due to low numbers of founding individuals [1, 2]. Early warning detection is key for managers to respond with best possible measures to prevent potential negative outcome to endemic fauna and biota [3, 4]. Traditional field surveys with visual inspection require taxonomic knowledge of the invading species and biotope knowledge combined with risk of dispersal to assess where to search [5]. Environmental DNA (eDNA), which is shed by all living organisms in the environment, is increasingly used for the detection of elusive or low abundant species and has been shown to be equally or more sensitive than traditional surveying methods [6, 7]. So far, the majority of developed eDNA single species detection methods have primarily focused on aquatic animals including mammalians [8, 9], fish [8, 1017] Molluscs [18, 19], crustacea [2022], amphibians [23, 24] reptiles [2527] and insects [2830]. Detection of aquatic plant eDNA has been scarce in comparison [31]. In the wild it was first demonstrated for Egeria densa and Hydrilla verticillata in small Japanese ponds (83–6000 m2) where the presence of the species was visually confirmed and compared to past distribution records [32, 33]. Another study successfully tested eDNA detection of H. verticillata in north American rivers and lakes [34] and three studies detected eDNA where the species had not yet been observed [31, 34, 35]. Several laboratory mesocosm experiments have tested the changes in aquatic plant eDNA over time, during and after introduction, and with and without grazers [3234]. However, to the best of our knowledge, no temporal or spatial studies have yet been conducted in the wild for studying potential seasonal variations and transportation of aquatic plants eDNA.

The Canadian pondweed Elodea canadensis Michaux, originates from North America and has colonized Europe at least since it was first recorded in Ireland in 1836 and Britain in 1842 [36]. The species was first observed in Norway in 1925, and has now spread to more than 100 southern Norwegian water bodies [37, 38] and has become the most widespread aquatic invasive macrophyte in Europe [39]. This is a rooted submerged flowering plant growing mostly in standing waters (canal, ditches, ponds, lakes). The species can produce 2–3 m long shoots and in clear water can grow down to 5–6 m depth [40]. The growing season starts in April–May and normally last until September-October, with biomass peak in July–August. However, in some lakes, e.g. Lake Steinsfjorden, the Elodea-stand can survive under ice-cover and collapse the following spring, and new growth develops from the decaying biomass [41, 42]. This species is dioecious, i.e. individual plants have only male or only female flowers, and in Europe male flowers are rarely seen suggesting the plant reproduction is mostly vegetative with overwintering buds and stem fragments [42]. In general E. canadensis shoots are sensitive to desiccation although apices and vegetative propagule may be more tolerant [43, 44]. These propagules can spread rapidly within lakes and downstream watercourses. Other vectors of dispersion can be by birds [45], but also most likely people through recreational boating, fish farming or angling [37, 46, 47].

This invasive aquatic plant is important for environmental management as it may affect the biodiversity and functioning of freshwater ecosystems where it grows in high abundance [40, 41, 48, 49].

In this study we developed molecular markers for E. canadensis, which we used for eDNA detection in two distinct studies and sites. The first study (site 1) is a spatial transect throughout an entire river catchment where eDNA results are compared with visual survey results and historical data to assess the spreading of the species. The aim of this first study is to assess how eDNA corroborates with visual results and to construct a gross estimate of eDNA disappearance during transportation. The second study (site 2) analyses eDNA signal strength through seasons at a location in a lake invaded by E. canadensis (time series) to assess how seasonality may affect eDNA signal strength of a sessile aquatic plant target.

We discuss how our findings may be useful for designing and interpreting aquatic plants eDNA surveys for management purposes.

Materials and methods

eDNA practices

Good practices for eDNA work [50] were implemented both in the field, for sample collection, filtration, and transport, as well as in the laboratory for DNA extraction and qPCR analysis. For optimum DNA preservation water samples were either transported on ice and processed with 24 h (site 1) or filtered on site (site 2). Virkon S (LANXESS Deutschland GmbH, Cologne, Germany) and 10% bleach solutions were used for neutralising DNA on surfaces including sample containers, field material and work benches in the laboratory. Prior knowledge of observed presence of the target species was used for ordering sampling in the field at site 1, collecting last known positive sites in the following way: # 8, 5, 4, 2, 1, 3, 7, 9, 6 & 10 (see Table 1.) Negative controls, minimum 2 and up to 6 per qPCR plate, were included in all runs. Due to the ecology of the targeted species, a sessile aquatic plant, allochthonous DNA is not foreseen to occur or interfere at the 2 studied sites. Calibration curves using standardized E. canadensis genomic DNA were used for quantification in fg/mL (S1 Fig). Quantification in target copy number using target qPCR products for the calibration curves was not used in order to reduce cross contamination risks. Probe-based qPCR technology was selected as recommended when using eDNA for single species detection [50]. Specificity of the E. canadensis probe assay was challenged both in-silico and in-vitro (see below for details). However, testing for possible inhibition was not performed albeit results indicate that no or little inhibition was present.

Table 1. Sampling information for the eDNA spatial transect (sampled 20.09.2018).

Study 1: eDNA spatial transect field survey

River Leira catchment description.

The river Leira is unregulated and drains a catchment area of 663 km2. The upper part of the catchment is covered by coniferous forest growing on rocks and moraine deposits. It is characterized by the presence of numerous large lakes, fast flowing and clear waters. The lower part of the catchment is dominated by agriculture, where the solid geology is covered by marine deposits including thick layers of clays. The density of the drainage network is much higher, the water is turbid with very high suspended sediment concentrations during high flow events [51]. The lower part of the Leira is meandering with many oxbow lakes and backwaters and discharges into the River Nitelva, just north of Lake Øyeren receiving the largest European freshwater delta from the River Glomma. Lake Øyeren is protected by the Ramsar convention and with over 40 species it hosts the highest species diversity of aquatic plants in Norway [52].

There were two known populations of E. canadensis in the catchment: Lake Nordbytjern (since 1989) half way up the catchment in the headwaters of a tributary (River Tveia); and a backwater Isakbekken (since 1982) in the lower part of the catchment where the river meanders [53] (Fig 1). Lake Nordbytjern is a small (surface area 0.2 km2), high alkalinity and mesotrophic lake. Elodea canadensis was also known to be present in the delta area (Nitelva, Svellet, Merkja) and adjacent catchments (Nitelva, Hersjøen and Risa) [53] (Fig 1).

Fig 1. Known distribution of Elodea canadensis in Norway and survey locations.

In AB The red triangular symbols represent localities with known presence of Elodea canadensis downloaded from Artsdatabanken (, and the green diamond symbols indicate the sites for the visual survey. In CD the blue circle symbols show water sample locations for eDNA analysis (with ID numbers #, see Table 1).

Spatial transect field survey description.

The design of this study took advantage of prior knowledge of presence of E. canadensis in the upper and lower parts of the catchment area. The important variations of turbidity across the studied area enabled qualitative assessment of its possible effect on eDNA detection. The clay rivers were generally not a suitable habitat for E. canadensis. We checked for presence of E. canadensis in the river at locations spaced every 5–7 km, which is a shorter distance than the spatial autocorrelation of aquatic plant composition in lowland rivers, i.e. 10 km [54]. The lower meandering part of the river Leira has several oxbow lakes, open backwaters and ditches. These stagnant or slow flowing water bodies are generally excellent habitat for aquatic plants and the most at risk of E. canadensis colonisation due to the proximity of the delta where E. canadensis is present (e.g. Møller and Rørdam [55]). This is also where the knock-on effect on biodiversity potentially is the highest.

We surveyed 23 sites by wading and/or snorkelling for 15–30 min (see S1 Table) selected for the likelihood of finding new populations of E. canadensis during summer 2018 (Fig 1). Water samples for eDNA analysis were collected in two 1 L prewashed plastic bottles with a wide neck and transported back to the lab in coolers with ice. Disinfection with Virkon S was carried out in between each sampling station. We took special care that no plant fragments adhered to our equipment.

We collected water samples from lakes and rivers of the Leira catchment area, an adjacent river (Nitelva, ID 10) and the delta area (Merkja, ID 8)–see Table 1 and Fig 1. Water samples were all collected on the 20th September 2018 under stable low flow conditions following a rainfall event. All the samples taken upstream of the marine clay deposits had clear water, but the samples from the lower part of the catchment were very turbid, mostly from suspended clay. The samples were collected just below the water surface (5–20 cm depth). Nitrile gloves were worn for collection of each sample and changed between sample sites. In the laboratory, bottles were wiped with 10% bleach before opening and filtered through a sterile 0.22 μm polyethersulfone disposable Sterivex GP filter with Luer-Lock (Millipore) with sterile disposable 60 mL Luer-Lock Tip syringes (Becton Dickinson). Briefly, the disposable 60 mL syringe is filled with the sample and fitted to the inlet female Luer-Lock of the Sterivex cartridge filter, to push through the water sample. The operation is repeated until the desired volume is filtered or when the capacity of the filter is reached, typically about 1 L for non-turbid samples. The remaining water in the cartridge is removed by using air in the syringe. DNA was further extracted from the Sterivex filters according to Spens, Evans [56].

Study 2: Lake Steinsfjorden eDNA time series field study

Lake Steinsfjorden is situated in the south-eastern lowland area of Norway, with surface area of 13.9 km2 and mean depth of 9.9 m. It is a moderately alkaline and slightly mesotrophic lake. The main watercourse upstream of Lake Steinsfjorden has been heavily infested with E. canadensis since the early 1960s, and E. canadensis was first observed in Steinsfjorden in 1978, in the southern and western parts of the lake [48]. From these localities, E. canadensis spread rapidly within the lake, until the distribution peaked in 1982 [57]. The distribution has remained relatively stable after that [58]. Water samples were collected once a month (May to October) at the surface (~1 m depth) and just above the bottom (~7 cm above, at ~3 m depth), at the same site (60.08175° N 10.337306° E (WGS84)). Due to issues with the pump only bottom samples were collected in June and July. This resulted in a total of ten samples covering the growth and initial decomposition period in Southern Norway with 6 sampling dates over a 5-month period. The eDNA water samples (5 L) were collected by pumping water directly from the lake onto glass-fibre filters (47 mm, 2 μm pore size, AP2504700 Millipore, Billerica, Massachusetts, USA) using a peristaltic pump (Masterflex E/S, Cole-Parmer, Vermon Hills, Illinois, USA) with Tygon tubing (Cole-Parmer) and an in-line filter holder (47 mm, Millipore). Ambient water was pumped through the tubing and filter holder to rinse the system between the bottom and surface samples. After field sampling, a 10% bleach solution was pumped through the tubing and filter holder and the tubing and filter holder was soaked in the bleach solution for 15 minutes to disinfect and remove eDNA traces. A 5% sodium thiosulfate solution was then used to rinse away the bleach. Due to high turbidity only 0,75 L sample was filtered above the bottom in October. Each filter was transferred to a 15 mL sterile falcon tube, stored on ice in a cooling box until transported to the laboratory within 12 hours, and frozen at -20°C. DNA was extracted from the glass-fibre filters using a CTAB extraction protocol, as described in [20]. These samples were initially collected as part of an eDNA study on freshwater crayfish that also inhabit the lake (unpublished) and thus the reason for a different sampling approach than in study 1. One of the benefits of eDNA sampling is the possibility to use the same sample to investigate the presence of several different organism.

Primer probe assay design and specificity

Alignments, primer and probe design based on the chloroplast intergenic spacer between the trnL and trnF genes, were made using Geneious R 10.1.3 ( and GeneDoc v2.7 softwares (see Table 2).

Specificity of the assay was challenged by testing possible cross amplification with the following macrophytes, many of which are commonly found in Norway: Potamogeton berchtoldii, P. obtusifolius, P. perfoliatus, P. friesii, P. pusillus and P. gramineus. All six tested macrophytes other than E. canadensis produced negative results. Although not tested, the assay should also be specific against Elodea nuttallii as the 3’ last eight nucleotides of the reverse primer EctrnL_R overlap a gap in the E. nuttallii sequence. Similarly, the probe EctrnL_P overlaps another eight-nucleotide gap in the E. nuttallii sequence (Fig 2), further increasing the specificity of the assay.

Fig 2. BLAST alignments.

Alignment of relevant Hydrocharitaceae sequences obtained by performing a BLAST search using the Elodea canadensis product sequence amplified from a locus on the intergenic spacer between trnL and trnF. Species specific oligonucleotides for E. canadensis and Egeria densa (Planch.) [32] are shown at the bottom.

This was completed with in-silico testing using the E. canadensis qPCR amplicon in a BLAST search [59]. The resulting species matches, all from the Hydrocharitaceae family, were aligned and are shown in Fig 2, confirming the specificity of the chosen oligonucleotides (see discussion). The species Hydrilla verticillata also had a partial match, although more divergent, and is included in S1 File along with GenBank access numbers and fasta files of the aligned products. The same sequences were used for designing the specific E. canadensis probe assay.


A CFX96 thermocycler (Bio-Rad, Hercules, CA, USA) was used to carry out qPCR amplifications with a final reaction volume of 25 μL containing 12.5 μL TaqMan Environmental Mix (ThermoFisher Scientific, Waltham, MA, US), 5 μL sample, 0.165 μL of forward primer (20 μM), 0.165 μL of reverse primer (20 μM), 1 μL probe (5 μM) (LGC Biosearch, Risskov, Denmark) and 5.25 μL sterile deionised water. Final concentrations are indicated in Table 2. A two-step cycling protocol was carried out with a 10 min denaturing step at 95°C, followed by 45 cycles of 95°C for 15 s and 58°C for 60 s. Reference DNA for E. canadensis was prepared as described previously [60]. Briefly, E. canadensis material was incubated for 5 minutes at 100°C with 600 μL sodium phosphate buffer (pH 8) in 1.5 ml Eppendorf tubes, and then transferred to a 2 ml cryopreservation tube with 0.5 g zirconium beads and 100 μL 25% sodium dodecyl sulphate added. Bead beating was performed in a Precellys 24 bead beater (Bertin, Technologies, Saint-Quentin, France) as following: 3 x 15 s at 6000 rpm, and 30 s at 6,00 rpm. The samples were then centrifuged (6 min, G = 13700) and DNA was further purified according to [61].

A 8.6 ng/μL stock solution was used to prepare a ten-fold serial dilution for qPCR calibration and calculation of amount of eDNA from each sampling location. The qPCR calibration curves had a 5-log base 10 linear range, a linear regression correlation coefficients (r2) greater than 0.99 and an efficiency equal or better than 97.0% (S1 Fig).

In addition to the positive control, negative extraction and blank qPCR controls were also added to each qPCR analysis. All qPCR samples were run in duplicate for Lake Steinsfjorden time series samples and in triplicate for the spatial transect samples.


River Leira eDNA spatial transect field survey

Presence of suspended clay in the lower part of the catchment had a marked incidence on the volumes that could be filtrated from about 1300 mL for the upstream samples down to 75 mL for the last downstream sample (see Fig 3). Elodea canadensis eDNA was detected in the sample from the source lake, with known presence of the aquatic plant, yielding almost 10 fg/mL. The detected eDNA quantities decreased in the next two consecutive sampling taken at the outlet of the lake and 1.4 km downstream, to thereafter show negative results for the next five downstream sampling locations (site 2–6, see Figs 1 & 4). The limit of detection was close to 0.1 fg/mL. The last two samples taken close to the mouth of River Leira (Nitelva and Merkja) showed eDNA concentrations exceeding 10 fg/mL despite turbid waters and low filtrated volumes.

Fig 3. eDNA spatial transect over the Leira catchment.

Numeration corresponds to Fig 1 and Table 1. Technical qPCR replicates are shown for each sampling site.

Fig 4. Elodea canadensis eDNA time series at Lake Steinsfjorden (one location).

No surface eDNA samples were collected on the 20 June and 18 July 2016. Filtered volume for all samples was 5000 mL apart for the 10th October 2016 bottom sample that used only 750 mL due to high turbidity. Technical qPCR replicates (parallels) are shown for each sampling date and depth.

Lake Steinsfjorden eDNA time series field study

All samples were collected at the same location (S2 Fig). All tested samples were positive for eDNA while all negative controls showed no amplification. Large seasonal variations were registered from around 1 fg/mL to over 1000 fg/mL eDNA quantities as shown in Fig 4. Bottom samples yielded more DNA than surface samples for the same date and location. The only turbid sample (October) that could not be filtered more than 750 mL, instead of 5000 mL for all others, also yielded the highest eDNA quantity. Two seasonal peaks were observed, one for the first sampling date in May and the highest for the last sampling date in October.


So far, most eDNA studies have focused on monitoring animal species, and only recently has this powerful method been applied to tracking aquatic plants. Typically, the eDNA is further analysed either by using species specific qPCR or traditional barcoding applied to water [31] for tracking invading or threatened species, or using metabarcoding for water [62] or soil samples [63] for global ecosystem biodiversity monitoring. In this study we have developed a specific probe assay for eDNA detection of the aquatic plant E. canadensis and used it to conduct a spatial transect field study as well as a time series field study. Variations in turbidity of the water samples along the spatial transect enabled evaluation of its possible interaction on eDNA detection.

E. canadensis specific probe assay

In an effort to stimulate the use of eDNA for the detection of invasive aquatic plants Scriver, Marinich [31] used three chloroplast markers for developing species specific assays and concluded that matK was the most likely to provide species specific nucleotides. However, no Elodea species were included for which Gantz, Renshaw [34] later developed assays detecting E. canadensis, E. nuttallii as well as Hydrilla verticillata. They also used the matK marker although the resulting assay that was developed could not differentiate between E. canadensis and E. nuttallii. However, a specific assay was reported using the genomic ITS1 marker. Finally, Fujiwara, Matsuhashi [33] used the intergenic spacer between the trnL and trnF genes for designing an assay for the specific detection of Egeria densa [33]. In the present study we also used the trnL–trnF intergenic spacer to design a probe assay specific for E. canadensis taking advantage of the two gaps present in the E. nuttallii sequences when aligned with the E. canadensis sequences. Although partly overlapping the Fujiwara, Matsuhashi [33] primers and probe, our assay should not cross amplify with E. densa because two SNPs are present in the 3’ end section of the reverse primer and one SNP is present in the probe (Fig 2). However, this was not tested. BLAST results showed three additional species belonging to the gender Ottelia which presented a sequence gap similar to E. nuttallii, precluding the possibility of annealing with the reverse primer of the E. canadensis assay (Fig 2). The higher copy number of chloroplast markers versus genomic markers usually make them better choices for assays requiring best possible sensitivity, as long as enough variation is present to enable the required specificity. We therefore believe our probe assay is well suited for the specific detection of E. canadensis from eDNA samples. An overview of species from the Hydrocharitaceae family with existing assays and range of distribution is given in S2 Table.

eDNA spatial transect study

Due to relatively fast degradation of DNA once it enters the environment [64] species detection can be considered recent as long as sediments have not been disturbed [65]. Due to this breakdown as well as dilution and adsorption, maximum range detection distance away from the target species, especially in running water, is not straight forward [66, 67]. Hence, biomass quantification estimates from eDNA should also take these aspects into consideration [10, 15]. The spatial transect of this study gives a first rough empirical estimate of E. canadensis eDNA persistence in a stream from a known source lake. The eDNA concentrations decreased (as expected) downstream from the lake with known presence of E. canadensis. The source population of E. canadensis in Lake Nordbytjern was situated in the south east corner of the lake, about 400±50 m from the outlet. The extent of Elodea population was about 100–500 m2 and not very dense (2017 boat survey with a bathyscope). At the time of survey E. canadensis presented no sign of decay, most of its leaves rather covered by calcium precipitate. Since eDNA was detected at the outlet and 1400 m downstream of the outlet, we can estimate the rate of disappearance of E. canadensis eDNA per unit distance in the lake and in the stream (S2 File). The rate of disappearance of E. canadensis eDNA was an order of magnitude loss over about 230 m in the lake and 1550 m in the stream. On average a detectable fragment of E. canadensis eDNA will travel 650 m in the stream (S2 File). These rates must be seen as first estimations to guide more detailed studies as for other organisms. In two other studies, eDNA from freshwater mussel beds (sessile organism) were detected 1000m downstream in a mesocosm [18] and 1700m downstream of a natural large aggregation [68], which is in the same range as the 1400m found in this study.

Water turbidity interaction with eDNA detection

Downstream samples of the spatial transect showed large differences in filtration volumes due to the presence of suspended clay in the lower parts of the river system. However, lower filtrated volumes due to turbidity may not necessarily hamper eDNA detection. Indeed, DNA bound on clay minerals (and other particles) can be more resistant to degradation [69] and this could explain the high eDNA concentrations obtained with the very small sample filtered volume performed at the mouth of the studied river. The high concentrations could also be partly explained by the known high densities of E. canadensis in the River Nitelva and the delta area (Merkja). The lake and stream outlet were situated above the marine clay deposits and had clear water, explaining the high volumes of water filtered at those sites (Fig 3). It would be interesting to measure the level of protection clay may provide to eDNA by assessing and comparing eDNA persistence from a source in either clear or clay-rich water systems. However, not all turbid waters will favour eDNA studies as they may also hinder proper eDNA evaluation of the studied water body [70].

eDNA time series field study

Variation through time of eDNA for a given species at a defined location is known to be dependent on various factors, in particular seasonal activity [71] as well as migration patterns for animals. Aquatic plants, with maybe the exception of floating plants, are less prone to geographic displacements. However, seasonal variations may still affect recovered eDNA concentrations in relation for example with growth activity, grazing or decay, as has also been seen for other taxa e.g. fish [15] and amphibians [71]. The time series performed in this study during the months of May through to October clearly shows seasonal variation of detected eDNA for E. canadensis at a given site. Relative quantities, from the lowest measure obtained in June, increased by more than three log base 10 to reach its maximum during the last sampling month in October. We believe this is explained by the plant’s biomass reaching its peak as well as onset of decaying during fall thereby releasing more DNA into the environment. Similar phenomena may explain the second smaller peak obtained at spring with the first sampling, due to plant survival under ice-covered lakes, in agreement with earlier studies also showing biomass collapse in spring [41, 42]. Differences between surface and bottom sampling at a same date and place were also consistently observed showing higher eDNA values with the bottom samples, including when turbidity limited the possible filtrated volume from 5 L to 0.75 L for the October bottom sample.


The autumn (October) seems to be the best period for sampling as plant biomass is at its peak with onset of decay, which showed in this study detected eDNA quantities about 1000 times higher than the lowest point observed when sampling during the month of June. Turbidity due to clay particles did not hamper eDNA detection and the rate of disappearance was in the range of one Log10 eDNA per km in the stream. These results are paramount for maximizing the efficiency of field surveys planning to map E. canadensis in possible new locations where quantities may be low, i.e. at the start of a colonization event. It is probable that many aquatic plants may show similar trends, which should always be taken into consideration when planning an eDNA survey.

Supporting information

S1 Fig. Calibration curve.

Calibration curve using tenfold serial dilution E. canadensis genomic DNA starting at 8.6 ng/μL with triplicate technical replicates.


S2 Fig. Lake Steinfjorden.

The sampling site (red symbol) is shown with the area cover of Elodea canadensis in 2004 (Mjelde et al 2012), similar to what was observed in 2017 (Demars, personal observation).


S1 Table. Field surveys.

Field surveys results for Elodea canadensis in the Leira catchment area. Geographic coordinates (EU89) in degrees.


S2 Table. Assays from the Hydrocharitaceae family.


S1 File. Fasta sequences from BLAST Alignments.

Alignment of relevant sequences obtained by performing a BLAST search using the Elodea canadensis product sequence located on the intergenic spacer between trnL and trnF. Species specific oligonucleotides are shown at the bottom.


S2 File. Assumptions and calculations of decay rates.



We would like to thank Andreas Ballot for providing the Elodea canadensis DNA reference material and Roar Brænden for helping to draw the maps.


  1. 1. Jerde CL, Mahon AR, Chadderton WL, Lodge DM. “Sight-unseen” detection of rare aquatic species using environmental DNA. Conservation Letters. 2011; 4(2):150–7.
  2. 2. Mahon AR, Jerde CL, Galaska M, Bergner JL, Chadderton WL, Lodge DM, et al. Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments. PLoS One. 2013; 8(3). ARTN e58316 WOS:000315637900124. pmid:23472178
  3. 3. Pluess T, Jarošík V, Pyšek P, Cannon R, Pergl J, Breukers A, et al. Which Factors Affect the Success or Failure of Eradication Campaigns against Alien Species? PLoS One. 2012; 7(10):e48157. pmid:23110197
  4. 4. Pluess T, Cannon R, Jarošík V, Pergl J, Pyšek P, Bacher S. When are eradication campaigns successful? A test of common assumptions. Biol Invasions. 2012; 14(7):1365–78.
  5. 5. Hellsten S, Willby N, Ecke F, Mjelde M, Phillips G, Tierney D. Water framework directive intercalibration technical report; Northern lake macrophyte ecological assessment methods. 2014 Report EUR 26513 EN; JRC88336.
  6. 6. Thomsen PF, Kielgast J, Iversen LL, Wiuf C, Rasmussen M, Gilbert MTP, et al. Monitoring endangered freshwater biodiversity using environmental DNA. Mol Ecol. 2012; 21(11):2565–73. WOS:000304389500004. pmid:22151771
  7. 7. Smart AS, Tingley R, Weeks AR, van Rooyen AR, McCarthy MA. Environmental DNA sampling is more sensitive than a traditional survey technique for detecting an aquatic invader. Ecol Appl. 2015; 25(7):1944–52. WOS:000362532600017. pmid:26591459
  8. 8. Andruszkiewicz EA, Starks HA, Chavez FP, Sassoubre LM, Block BA, Boehm AB. Biomonitoring of marine vertebrates in Monterey Bay using eDNA metabarcoding. PLoS One. 2017; 12(4):e0176343. pmid:28441466
  9. 9. Ushio M, Fukuda H, Inoue T, Makoto K, Kishida O, Sato K, et al. Environmental DNA enables detection of terrestrial mammals from forest pond water. Mol Ecol Resour. 2017; 17(6):e63–e75. pmid:28603873
  10. 10. Takahara T, Minamoto T, Yamanaka H, Doi H, Kawabata Z. Estimation of Fish Biomass Using Environmental DNA. PLoS One. 2012; 7(4). ARTN e35868 WOS:000305349100037. pmid:22563411
  11. 11. Minamoto T, Uchii K, Takahara T, Kitayoshi T, Tsuji S, Yamanaka H, et al. Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio. Mol Ecol Resour. 2017; 17(2):324–33. pmid:27487846
  12. 12. Fossøy F, Thaulow J, Anglès d'Auriac M, Brandsegg H, Sivertsgård R, Mo TA, et al. Bruk av miljø-DNA som supplerende verktøy for overvåkning og kartlegging av fremmed ferskvannsfisk. NINA, 2018 1586 Contract No.: 1586.
  13. 13. Sassoubre LM, Yamahara KM, Gardner LD, Block BA, Boehm AB. Quantification of Environmental DNA (eDNA) Shedding and Decay Rates for Three Marine Fish. Environmental Science & Technology. 2016; 50(19):10456–64. pmid:27580258
  14. 14. Klymus KE, Richter CA, Chapman DC, Paukert C. Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix. Biological Conservation. 2015; 183:77–84. WOS:000349878700010.
  15. 15. Doi H, Inui R, Akamatsu Y, Kanno K, Yamanaka H, Takahara T, et al. Environmental DNA analysis for estimating the abundance and biomass of stream fish. Freshwater Biol. 2017; 62(1):30–9.
  16. 16. Deutschmann B, Müller A-K, Hollert H, Brinkmann M. Assessing the fate of brown trout (Salmo trutta) environmental DNA in a natural stream using a sensitive and specific dual-labelled probe. Science of The Total Environment. 2019; 655:321–7. pmid:30471600
  17. 17. Hulley EN, Tharmalingam S, Zarnke A, Boreham DR. Development and validation of probe-based multiplex real-time PCR assays for the rapid and accurate detection of freshwater fish species. PLoS One. 2019; 14(1):e0210165. Epub 2019/01/31. pmid:30699146.
  18. 18. Sansom BJ, Sassoubre LM. Environmental DNA (eDNA) shedding and decay rates to model freshwater mussel eDNA transport in a river. Environmental Science & Technology. 2017; 51(24):14244–53. WOS:000418625900027. pmid:29131600
  19. 19. Bc Stoeckle, Kuehn R, Geist J. Environmental DNA as a monitoring tool for the endangered freshwater pearl mussel (Margaritifera margaritifera L.): a substitute for classical monitoring approaches? Aquatic Conservation: Marine and Freshwater Ecosystems. 2015:n/a-n/a.
  20. 20. Strand DA, Johnsen SI, Rusch JC, Agersnap S, Larsen WB, Knudsen SW, et al. Monitoring a Norwegian freshwater crayfish tragedy: eDNA snapshots of invasion, infection and extinction. J Appl Ecol. 2019; 0(0).
  21. 21. Rice CJ, Larson ER, Taylor CA. Environmental DNA detects a rare large river crayfish but with little relation to local abundance. Freshwater Biol. 2018; 63(5):443–55.
  22. 22. Mauvisseau Q, Coignet A, Delaunay C, Pinet F, Bouchon D, Souty-Grosset C. Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds. Hydrobiologia. 2018; 805(1):163–75. WOS:000415692400011.
  23. 23. Goldberg CS, Pilliod DS, Arkle RS, Waits LP. Molecular Detection of Vertebrates in Stream Water: A Demonstration Using Rocky Mountain Tailed Frogs and Idaho Giant Salamanders. PLoS One. 2011; 6(7). WOS:000293175100047. pmid:21818382
  24. 24. Takahashi MK, Meyer MJ, Mcphee C, Gaston JR, Venesky MD, Case BF. Seasonal and diel signature of eastern hellbender environmental DNA. J Wildl Manage. 2018; 82(1):217–25. WOS:000417629600021.
  25. 25. Piaggio AJ, Engeman RM, Hopken MW, Humphrey JS, Keacher KL, Bruce WE, et al. Detecting an elusive invasive species: a diagnostic PCR to detect Burmese python in Florida waters and an assessment of persistence of environmental DNA. Mol Ecol Resour. 2013. pmid:24119154.
  26. 26. Davy CM, Kidd AG, Wilson CC. Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles. PLoS One. 2015; 10(7):e0130965. pmid:26200348
  27. 27. Reinhardt T, van Schingen M, Windisch HS, Nguyen TQ, Ziegler T, Fink P. Monitoring a loss: Detection of the semi-aquatic crocodile lizard (Shinisaurus crocodilurus) in inaccessible habitats via environmental DNA. Aquatic Conservation: Marine and Freshwater Ecosystems. 0(0).
  28. 28. Valentin RE, Fonseca DM, Nielsen AL, Leskey TC, Lockwood JL. Early detection of invasive exotic insect infestations using eDNA from crop surfaces. Frontiers in Ecology and the Environment. 2018; 16(5):265–70.
  29. 29. Doi H, Katano I, Sakata Y, Souma R, Kosuge T, Nagano M, et al. Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem. Royal Society Open Science. 2017; 4(7):170568. pmid:28791177
  30. 30. Klymus KE, Marshall NT, Stepien CA. Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes. PLoS One. 2017; 12(5):e0177643. pmid:28542313
  31. 31. Scriver M, Marinich A, Wilson C, Freeland J. Development of species-specific environmental DNA (eDNA) markers for invasive aquatic plants. Aquat Bot. 2015; 122:27–31. WOS:000352172400005.
  32. 32. Matsuhashi S, Doi H, Fujiwara A, Watanabe S, Minamoto T. Evaluation of the environmental DNA method for estimating distribution and biomass of submerged aquatic plants. PLoS One. 2016; 11(6):e0156217. WOS:000377824800014. pmid:27304876
  33. 33. Fujiwara A, Matsuhashi S, Doi H, Yamamoto S, Minamoto T. Use of environmental DNA to survey the distribution of an invasive submerged plant in ponds. Freshw Sci. 2016; 35(2):748–54. WOS:000376471600025.
  34. 34. Gantz CA, Renshaw MA, Erickson D, Lodge DM, Egan SP. Environmental DNA detection of aquatic invasive plants in lab mesocosm and natural field conditions. Biol Invasions. 2018; 20(9):2535–52. WOS:000441112500018.
  35. 35. Kuzmina ML, Braukmann TW, Zakharov EV. Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds. Applications in Plant Sciences. 2018; 6(5):e01155. pmid:30131897
  36. 36. Simpson DA. A short history of the introduction and spread of Elodea Michx in the British Isles (1984) Watsonia. 1984; 15:1–14.
  37. 37. Mjelde M, Berge D, Edvatdsen H. Kunnskapsgrunnlag for handlingsplan mot vasspest (Elodea canadensis) og smal vasspest (Elodea nuttallii) i Norge. Norwegian Institute for Water Research, 2012 Contract No.: 6416–2012.
  38. 38. Mjelde M. Vasspest Elodea canadensis. Artsdatabank faktaark [Internet]. 2012. Available from:
  39. 39. Hussner A. Alien aquatic plant species in European countries. Weed Research. 2012; 52(4):297–306.
  40. 40. Mjelde M, Lombardo P, Berge D, Johansen SW. Mass invasion of non-native Elodea canadensis Michx. in a large, clear-water, species-rich Norwegian lake–impact on macrophyte biodiversity. Ann Limnol—Int J Lim. 2012; 48(2):225–40.
  41. 41. Rørslett B, Berge D, Johansen SW. Mass invasion of Elodea canadensis in a mesotrophic, South Norwegian lake—impact on water quality. SIL Proceedings, 1922–2010. 1985; 22(5):2920–6.
  42. 42. Spicer KW, Catling PM. The biology of Canadian weeds. 88. Elodea canadensis Michx. Canadian Journal of Plant Science. 1988; 68(4):1035–51.
  43. 43. Barnes MA, Jerde CL, Keller D, Chadderton WL, Howeth JG, Lodge DM. Viability of Aquatic Plant Fragments following Desiccation. Invasive Plant Science and Management. 2013; 6(2):320–5. Epub 2017/01/20.
  44. 44. Coughlan NE, Cuthbert RN, Kelly TC, Jansen MAK. Parched plants: survival and viability of invasive aquatic macrophytes following exposure to various desiccation regimes. Aquat Bot. 2018; 150:9–15.
  45. 45. Coughlan NE, Kelly TC, Jansen MAK. "Step by step": high frequency short-distance epizoochorous dispersal of aquatic macrophytes. Biol Invasions. 2017; 19(2):625–34. WOS:000394152300014.
  46. 46. Anderson LG, White PCL, Stebbing PD, Stentiford GD, Dunn AM. Biosecurity and vector behaviour: evaluating the potential threat posed by anglers and canoeists as pathways for the spread of invasive non-native species and pathogens. PLoS One. 2014; 9(4):e92788. WOS:000334339000020. pmid:24717714
  47. 47. Sutcliffe C, Quinn CH, Shannon C, Glover A, Dunn AM. Exploring the attitudes to and uptake of biosecurity practices for invasive non-native species: views amongst stakeholder organisations working in UK natural environments. Biol Invasions. 2018; 20(2):399–411. WOS:000426065700011.
  48. 48. Rørslett B, Berge D, Johansen SW. Lake enrichment by submersed macrophytes: A Norwegian whole-lake experience with Elodea canadensis. Aquat Bot. 1986; 26:325–40.
  49. 49. Carpenter SR, Lodge DM. Effects of submersed macrophytes on ecosystem processes. Aquat Bot. 1986; 26:341–70.
  50. 50. Goldberg CS, Turner CR, Deiner K, Klymus KE, Thomsen PF, Murphy MA, et al. Critical considerations for the application of environmental DNA methods to detect aquatic species. Methods in Ecology and Evolution. 2016:n/a-n/a.
  51. 51. Bogen J, Sandersen F. Sedimentkilder, erosjonsprosesser og sedimenttransport i Leira-vassdraget på Romerike. Delrapport i prosjektet: Forurensing som følge av leireerosjon og betydningen av erosjonsforebyggende tiltak. NVE, 1991.
  52. 52. Rørslett B. Miljøfaglige undersøkelser i Øyeren 1994–2000 Fagrapport: Vannbotanikk Norwegian Institute for Water Research, 2002.
  53. 53. Demars B, Anglès d’Auriac MB, Thaulow J, Bræenden R, Mjelde M. Kartlegging av vasspest i Vannområde Leira-Nitelva 2018. Norwegian Institute for Water Research, 2018 Contract No.: 73-03-2018.
  54. 54. Demars BOL, Wiegleb G, Harper DM, Broring U, Brux H, Herr W. Aquatic Plant Dynamics in Lowland River Networks: Connectivity, Management and Climate Change. Water. 2014; 6(4):868–911. WOS:000335761800008.
  55. 55. Møller TR, Rørdam CP. Species Numbers of Vascular Plants in Relation to Area, Isolation and Age of Ponds in Denmark. Oikos. 1985; 45(1):8–16.
  56. 56. Spens J, Evans AR, Halfmaerten D, Knudsen SW, Sengupta ME, Mak SST, et al. Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter. Methods in Ecology and Evolution. 2016; (8):635–45.
  57. 57. Berge D, al. e. Vasspest. Problem og ressurs. Sammenfattende sluttrapport fra vasspestprosjektene. Norwegian Institute for Water Research, 1989 NIVA-rapport O-86238.
  58. 58. Mjelde M, Lombardo P, Berge D, Johansen SW. Mass invasion of non-native Elodea canadensis Michx. in a large, clear-water, species-rich Norwegian lake–impact on macrophyte biodiversity. Annales de Limnologie—International Journal of Limnology. 2012; 48(02):225–40.
  59. 59. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389–402. pmid:9254694
  60. 60. Schneider SC, Nowak P, Von Ammon U, Ballot A. Species differentiation in the genus Chara (Charophyceae): considerable phenotypic plasticity occurs within homogenous genetic groups. European Journal of Phycology. 2016; 51(3):282–93.
  61. 61. Hagman CHC, Ballot A, Hjermann DO, Skjelbred B, Brettum P, Ptacnik R. The occurrence and spread of Gonyostomum semen (Ehr.) Diesing (Raphidophyceae) in Norwegian lakes. Hydrobiologia. 2015; 744(1):1–14. WOS:000346182100001.
  62. 62. Kuzmina ML, Braukmann TWA, Zakharov EV. Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds. Applications in plant sciences. 2018; 6(5):e01155–e. pmid:30131897
  63. 63. Fahner NA, Shokralla S, Baird DJ, Hajibabaei M. Large-Scale Monitoring of Plants through Environmental DNA Metabarcoding of Soil: Recovery, Resolution, and Annotation of Four DNA Markers. PLoS One. 2016; 11(6):e0157505. pmid:27310720
  64. 64. Dejean T, Valentini A, Duparc A, Pellier-Cuit S, Pompanon F, Taberlet P, et al. Persistence of Environmental DNA in Freshwater Ecosystems. PLoS One. 2011; 6(8). WOS:000293773300026. pmid:21858099
  65. 65. Turner CR, Uy KL, Everhart RC. Fish environmental DNA is more concentrated in aquatic sediments than surface water. Biological Conservation. 2015; 183:93–102.
  66. 66. Deiner K, Altermatt F. Transport distance of invertebrate environmental DNA in a natural river. PLoS One. 2014; 9(2):e88786. WOS:000331258100089. pmid:24523940
  67. 67. Jane SF, Wilcox TM, McKelvey KS, Young MK, Schwartz MK, Lowe WH, et al. Distance, flow and PCR inhibition: eDNA dynamics in two headwater streams. Mol Ecol Resour. 2015; 15(1):216–27. WOS:000346699100020. pmid:24890199
  68. 68. Wacker S, Fossøy F, Larsen BM, Brandsegg H, Sivertsgård R, Karlsson S. Downstream transport and seasonal variation in freshwater pearl mussel (Margaritifera margaritifera) eDNA concentration. Environmental DNA. 2019; 0(0).
  69. 69. Alvarez AJ, Khanna M, Toranzos GA, Stotzky G. Amplification of DNA bound on clay minerals. Mol Ecol. 1998; 7(6):775–8.
  70. 70. Egeter B, Peixoto S, Brito JC, Jarman S, Puppo P, Velo-Antón G. Challenges for assessing vertebrate diversity in turbid Saharan water-bodies using environmental DNA. Genome. 2018; 61(11):807–14. pmid:30312548
  71. 71. Buxton AS, Groombridge JJ, Griffiths RA. Seasonal variation in environmental DNA detection in sediment and water samples. PLoS One. 2018; 13(1):e0191737. pmid:29352294