Fig 1.
Flowchart showing detail protocol for conventional method and DNA based detection using LAMP.
Fig 2.
(a) Schematic and (b) actual view: isothermal amplification and detection assembly (c) signal at detector at various bacterial concentrations.
Fig 3.
Calibration curve: Intensity at 15 minutes (a) LAMP assay versus initial concentration from 5 to 500 CFU/mL along with (b) post-four-hour incubation. For post-incubated samples, the approximate CFU/mL count was obtained by a count of cultured bacteria on agar plates as shown by red lines. The linear regime is shown by the dotted lines.
Fig 4.
(a) Cross-reactivity of primers against other bacterial species spiked in sterile blood culture media (final concentration of 106 CFU/mL) (b) positive (S. typhi) and negative control at pre-and post-four-hour incubation.
Fig 5.
Detection of S. typhi by Miod from human blood samples in the medical institute.
The corresponding data are shown only for representative six clinical samples.
Table 1.
Results of culture and biochemical tests of the representative six clinical samples.
Table 2.
A comparative analysis of conventional method and Miod detection of S. typhi.
Table 3.
Comparison of Miod with newly developed LAMP based amplification techniques.