Fig 1.
Acclimation of Arabidopsis thaliana ecotype Col plants to salt, heat and a combination of salt and heat stress.
(A) Growth parameters, chlorophyll content and survival of plants subjected to salt, heat and a combination of salt and heat stress. (B) Representative images of plants subjected to the different stresses. * or **, Student’s t test significant at *P < 0.05 or **P < 0.01 compared to control (n = 30). Error bars represent SD.
Fig 2.
Na+ and K+ ion content, and Na+/K+ ratio in leaves of Arabidopsis plants subjected to salt, heat and a combination of salt and heat stress.
**, Student’s t test significant at *P<0.01 compared to control (n = 27). Error bars represent SE.
Fig 3.
Transcriptomic analysis of Arabidopsis plants subjected to salt, heat and a combination of salt and heat stress.
(A) Venn diagram showing the overlap between transcripts significantly up- or down-regulated in response to salt, heat and a combination of salt and heat stress. (B) Representation of ROS-, hormone-, and abiotic/biotic stress- response transcripts within the group of transcripts that are significantly up-regulated in response to the combination of salt and heat stress. (C) GO annotation of the transcripts specifically up-regulated in response to a combination of salt and heat stress.
Fig 4.
Response of Arabidopsis ecotype Ler plants to salt, heat and a combination of salt and heat stress.
(A) Growth parameters, chlorophyll content and survival of plants subjected to salt, heat and a combination of salt and heat stress. (B) Representative images of plants subjected to the different stresses. * or **, Student’s t test significant at *P < 0.05 or **P < 0.01 compared to control (n = 30). Error bars represent SD.
Fig 5.
Response of aba1 plants to salt, heat and a combination of salt and heat stress.
(A) Growth parameters and survival of plants subjected to salt, heat and a combination of salt and heat stress. (B) Representative images of plants subjected to the different stresses. * or **, Student’s t test significant at *P < 0.05 or **P < 0.01 compared to WT (n = 30). Error bars represent SD. Absolute values for the graphs shown in (A) are presented in S9 Fig.
Fig 6.
Response of abi1 plants to salt, heat and a combination of salt and heat stress.
(A) Growth parameters and survival of plants subjected to salt, heat and a combination of salt and heat stress. (B) Representative images of plants subjected to the different stresses. * or **, Student’s t test significant at *P < 0.05 or **P < 0.01 compared to WT (n = 30). Error bars represent SD. Absolute values for the graphs shown in (A) are presented in S10 Fig.
Fig 7.
Specificity of stress combination-specific transcripts.
(A) Overlap between transcripts specifically and significantly up-regulated in response to a combination of salt and heat stress, and transcripts specifically and significantly up-regulated in response to other stress combinations. (B) Venn diagram showing the overlap between transcripts specifically and significantly up-regulated in response to a combination of salt and heat stress, and transcripts specifically and significantly up-regulated in response to a combination of drought and heat stress. References used for the meta-analysis are [7, 8, 52].