Table 1.
Heading date variation and significance analyses of 28 CASLs and Triticum aestivum var. Chinese Spring (CS).
Table 2.
General heading date statistics for the four F2 populations and their two parents.
Fig 1.
Distribution of heading dates in four wheat F2 populations.
M: Middle value; CS: Chinese Spring; 2BS: CASL2BS; 3AL: CASL3AL; E1:CASL2BS×CS (Planting time: 11/10/2012); E2: CASL2BS×CS (Plant time:1/26/2013); E3: CASL3AL×CASL 2BS (Planting time: 11/10/2012); E4:CASL3AL×CASL 2BS (Planting time: 1/26/2013).
Table 3.
Segregation ratio analysis of the four F2 populations.
Fig 2.
Identification of the Ppd-B1 gene on chromosome arm 2BS by SSCP analysis of 28 CASLs (#1-#28) and their parents.
C: CS; and T: TDIC140. The black arrow indicates the TDIC band in CASL2BS (#17).
Fig 3.
Linkage map of wheat chromosome 2BS constructed using segregation data from four F2 populations and locations of QTL related to heading date.
Table 4.
QTL locations, related maximum LOD values and their explanation of phenotypic variation for heading date detected in the four mapping populations.
Fig 4.
TaqMan® estimation of Ppd-B1 copy number in Chinese Spring (CS), TDIC140 and CASL2BS.
Solid circles are genotypes known to have a photoperiod sensitive (Ppd-B1b) allele and open circles are genotypes known to have a day neutral (Ppd-B1a) allele. Copy number was estimated based on the Ppd-B1/Internal Positive Control (IPC) signal ratio. Means and standard deviations of three measurements are shown. T-tests show the classes that differed significantly from one another (*p<0.001).
Fig 5.
Relative transcript levels of three flowering genes in CS (black triangles), TDIC140 (black diamonds) and CASL 2BS (black squares) using time-course RT-qPCR.
Sampling was performed on 30-day-old seedlings starting at 6:00 AM (Time = 0) of the second day under long days (15 h light / 9 h dark). Leaves were harvested every 3 hrs from at least three plants per time point per genotype (biological replicates). Error bars indicate standard error of the mean. A sterisks show probabilities that the respective alleles in CS, CASL2BS and TDIC140 have expression levels that are not significantly different, based upon T tests (* p<0.001).