Table 1.
Potential Asteraceae host plants screened in this study for the presence of Ozirhincus gall midges.
Fig 1.
Phylogenetic tree of Ozirhincus Rondani based on Bayesian analysis of partial sequence of the cytochrome oxidase subunit I (COI) and ribosomal RNA16S mitochondrial genes.
Support values are shown next to nodes, above branches. Character states representing proboscis length as suggested by the ancestral states analysis are shown below branches (in square brackets). Letters and numbers following species name refer to collecting localities and dates (details in Table 2). Colors correspond to host-plant genera.
Fig 2.
a-c. Female on Tanacetum vulgare inflorescence (photos: Hedy Jansen); d. Tanacetum vulgare flowers containing O. hungaricus larvae (upper row), and normal flowers (lower row).
Fig 3.
a. Ozirhincus anthemidis, male; b. O. hungaricus, male; c. O. millefolii, female; d. O. longicollis, female; e. O. longicollis, male, showing typical shape of flagellomeres.
Fig 4.
a. Ozirhincus anthemidis, frontal; b. O. anthemidis, lateral; c. O. hungaricus, frontal; c. O. hungaricus, lateral; e. O. longicollis, frontal; f. O. millefolii, frontal.
Fig 5.
a. Ozirhincus anthemidis, male apical flagellomeres, setae not shown; b. O. anthemidis, male flagellomeres, some setae shown; c. O. anthemidis, female flagellomeres; d. O. longicollis, male apical flagellomeres; e. O. longicollis, male (left), female (right); f. O. millefolii, male (left), female (center and right). Scale bars = 0.1 mm.
Fig 6.
a. Ozirhincus anthemidis; b. O. hungaricus. c-e. O. anthemidis, fifth tarsomere, claw and acropod. c. Lateral; d. Lateral, showing both claws; e. Ventral. Lb–Labella, Lr–Labrum, Pl–Palp. Scale bars = 0.1 mm.
Fig 7.
a. Ozirhincus anthemidis, lateral; b. O. anthemidis, ovipositor, lateral; c. O. anthemidis, 7th tergite; d. O. hungaricus, 7th tergite; e. O. longicollis, 7th tergite; f. O. millefolii, 7th tergite. Al–Apical lamella, Dlp–Dorsolateral plate, Lgs–Lateral group of setae on eighth segment. Scale bars = 0.1 mm, except for Fig 28 = 0.5 mm.
Fig 8.
Male abdomen, Ozirhincus anthemidis.
a. Post abdomen and terminalia, lateral; b. Terminalia, dorsal, setation shown on right gonopod; c. Terminalia, lateral, one gonopod removed; d. Terminalia, ventral, showing mediobasal lobes and aedeagus; e. Gonostylus, dorsal (top), ventral (bottom). Aed–Aedeagus, Cer–cercus, Dl–Dorsal part of mediobasal lobe, Gs–Gonostylus, Gx–Gonocoxite, Vl–Ventral part of mediobasal lobe. Scale bars = 0.1 mm.
Fig 9.
a. O. anthemidis, head; b. O. anthemidis, spatula and associated papillae; c. O. anthemidis, terminal abdominal segment; d. O. hungaricus, terminal abdominal segment. e. Spatula and associated papillae, O. hungaricus; f. Spatula and associated papillae, O. longicollis; g. Spatula and associated papillae, O. millefolii; h. Variation of spatula shape in O. anthemidis; i. Variation of spatula shape in O. hungaricus. Lp–Lateral papillae, Sp–Sternal papilla, Vp–Ventral papilla. Scale bars = 0.1 mm.
Table 2.
Samples used for analysis of the COI and 16S mitochondrial genes, with GenBank accession numbers.