Table 1.
Monoclonal antibody reagents used in the study.
Fig 1.
Gating strategy for activation panel (upper) and memory panel (lower).
The sequential gating to CD3+ T cells is the same for both panels as shown in the upper row for each panel. Gating on CD4+ and CD8+ T cells is shown in the lower left graph in each panel. Markers of interest for each panel for CD4+ and CD8+ T cells are shown in the remaining plots.
Table 2.
Study Populations.
Fig 2.
Fluorescence-minus-one (FMO) controls for the activation panel.
Shown is an example from one experiment at one site. The upper graphs show the FMO for APC CD69, the middle for PE CD38, and the lower for FITC HLA-DR. The three specimen types are shown with MMC on the left, PBMC in the middle and qPBMC on the right. For each, graphs are paired with the full stain on the left and the FMO on the right. Note that the FMO defines the lower limit of the gate; often the gate is placed higher.
Fig 3.
qPBMC Levey-Jennings plots for the CD4+ percentage of CD3+ cells and the CD8+ CD38+DR+ percentage of CD8+ cell populations from the activation panel and the CD4+ percentage of CD3+ cells and the CD27-CD45RA- percentage of CD8+ cell populations from the memory panel.
Each experiment (subject) is shown on the x-axis. The bold black line shows the mean, and the dotted red line shows +/- 3 SD from the mean. Green and yellow lines are for 1 and 2 SD.
Table 3.
qPBMC Comparisons.
Fig 4.
Selected qPBMC data for CD4+, CD8+, CD4+CCR5+, CD8+CCR5+, CD4+CD38+DR+, and CD8+CD38DR+ T cells (boxplots showing the median, quartiles, and range for each cell population).
Table 4.
PBMC Comparisons.
Table 5.
MMC Comparisons.
Fig 5.
Selected PBMC data for CD4+, CD8+, CD4+CCR5+, CD8+CCR5+, CD4+CD38+DR+, and CD8+CD38DR+ T cells (boxplots showing the median, quartiles, and range for each cell population).
Fig 6.
Selected MMC data for: CD4+, CD8+, CD4+CCR5+, CD8+CCR5+, CD4+CD38+DR+, and CD8+CD38DR+ T cells (boxplots showing the median, quartiles, and range for each cell population).