Table 1.
Origin of the Microcyclus ulei isolates used in the phylogenetic study.
Figure 1.
Bayesian analysis showing the phylogenetic relationships of Microcyclus ulei based on the LSU sequence alignment.
Bayesian posterior probabilities are given at the nodes and coded according to the colored scale bar. The black line scale bar shows 0.2 expected changes per site. The tree was rooted with Aspergillus niger.
Figure 2.
Bayesian analysis showing the phylogenetic relationships of Microcyclus ulei based on the mtSSU sequence alignment.
Bayesian posterior probabilities are given at the nodes and coded according to the colored scale bar. The black line scale bar shows 0.2 expected changes per site. The tree was rooted with Aspergillus niger.
Figure 3.
Bayesian analysis showing the phylogenetic relationships of Microcyclus ulei based on the MCM7 sequence alignment.
Bayesian posterior probabilities are given at the nodes and coded according to the colored scale bar. The black line scale bar shows 0.2 expected changes per site. The tree was rooted with Aspergillus niger.
Figure 4.
Phylogenetic relationships of Microcyclus ulei based on the combined ITS, EF-1α and ACT sequences alignment.
Bayesian posterior probabilities are given at the nodes and coded according to the colored scale bar. The black line scale bar shows 0.08 expected changes per site. The tree was rooted with Passalora eucalypti.
Figure 5.
Pleomorphic development of the life cycle of Pseudocercospora ulei.
Weighted average based on the score of conidial sporulation intensity and spermogonia and ascostromata density during the leaf development of RO38 rubber clone grown under field conditions. Assessments were made from December 15, 2011 to February 24, 2012 (A); and from September 19 to December 3, 2012 (B).
Figure 6.
Hypothetical life cycle of Pseudocercospora ulei.
A. Asexual morph with conidiophores and conidia (Bar = 35µm) and conidiogenous cells with conidia at different stages of conidial formation. Pictures: Lesions to which the asexual morph is associated (left) and close-up of leaf bearing typical lesions (right). B. Spermogonial morph with stroma, spermogonia (Bar = 30 µm) and spermatia (Bar = 7 µm). Pictures: Lesions to which the spermogonial morph is associated (left), and close-up of the same lesions (right). C. Sexual morph with stroma, pseudothecia, asci and ascospores (Bar = 60µm). Pictures: Lesions to which the sexual morph is associated (left), and close-up of stromata (right). Dotted arrows indicate that both ascospores and conidia can infect young leaves.