Figure 1.
Affinities of the HAs of the H3NA- viruses for sialylglycopolymers.
Binding curves were fitted with the two models MM and MM2 for A/Moscow/10/99, A/Lyon-CHU/26430/03 and A/Reunion/586/04 viruses with 6′SLN receptors (A) and 3′SL receptors (B). The values of the dissociation constant (Kd) of the three viruses for 6′SLN receptors (C) and 3′SL receptors (D) calculated from the two models (MM and MM2) are indicated. The values correspond to the mean +/– standard deviation calculated from three independent assays. The Bayesian Information Criterion (BIC) is indicated for each regression.
Table 1.
Recombinant viruses produced by reverse genetics and their growth characteristics after two passages in MDCK cells.
Figure 2.
Kinetics of virus growth experiments in MDCK cells.
P2 MDCK viruses were used to infect MDCK cells at a m.o.i of 0.001. Supernatants were harvested at the indicated times. The TCID50/50 µl values correspond to the mean +/– standard deviation calculated from two independent assays.
Table 2.
Experiments done on H3NA- viruses.