Table 1.
Oligonucleotide primers and probes used in this study.
Figure 1.
Amplification curves for multiplex real-time PCR assay.
Figure 1 A. Real-time amplification curves for M. caprae (circle) using lepA gene in Cyan500 channel (450–500). Figure 1 B Amplification curves for M. caprae (circle), M. bovis (triangles) and M. bovis BCG (diamonds) using the lpqT gene in FAM channel (483–533). Figure 1 C Amplification curves for all members of the MTC with the exception of M. bovis BCG (diamonds) and M. microti in the HEX channel (523–568). Figure 1 D Amplification curves for IAC in Cy5 channel (615–670) with the no template control highlighted with stars through amplification curve.
Table 2.
Result interpretation table.