Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing
Fig 2
Automatic dispenser offset compensation (AOC) by measuring droplet placement position before / during the cell isolation process.
(A) For this, droplets are dispensed on a glass slide that is imaged by a digital camera. (B) The actual droplet position is extracted from the image data by image processing with openCV. (C) displays the binary image after thresholding. The algorithm automatically calculates the correct dispensing position to target the center of the microwell.